Sex chromosome complement contributes to sex differences in coxsackievirus B3 but not influenza A virus pathogenesis

被引：64

作者：

Robinson D.P. ^{[1
]}

Huber S.A. ^{[2
]}

Moussawi M. ^{[2
]}

Roberts B. ^{[2
]}

Teuscher C. ^{[3
]}

Watkins R. ^{[4
]}

Arnold A.P. ^{[4
]}

Klein S.L. ^{[1
]}

机构：

[1] W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore

[2] Department of Pathology, University of Vermont, Burlington

[3] Department of Medicine, University of Vermont, Burlington

[4] Department of Integrative Biology and Physiology, Brain Research Institute, University of California, Los Angeles

来源：

Biology of Sex Differences | / 2卷 / 1期

基金：

美国国家卫生研究院;

关键词：

Myocarditis; Gonadal Hormone; Experimental Autoimmune Encephalitis; CVB3 Infection; Four Core Genotype;

D O I：

10.1186/2042-6410-2-8

中图分类号：

学科分类号：

摘要：

Background: Both coxsackievirus B3 (CVB3) and influenza A virus (IAV; H1N1) produce sexually dimorphic infections in C57BL/6 mice. Gonadal steroids can modulate sex differences in response to both viruses. Here, the effect of sex chromosomal complement in response to viral infection was evaluated using four core genotypes (FCG) mice, where the Sry gene is deleted from the Y chromosome, and in some mice is inserted into an autosomal chromosome. This results in four genotypes: XX or XY gonadal females (XXF and XYF), and XX or XY gonadal males (XXM and XYM). The FCG model permits evaluation of the impact of the sex chromosome complement independent of the gonadal phenotype. Methods: Wild-type (WT) male and female C57BL/6 mice were assigned to remain intact or be gonadectomized (Gdx) and all FCG mice on a C57BL/6 background were Gdx. Mice were infected with either CVB3 or mouseadapted IAV, A/Puerto Rico/8/1934 (PR8), and monitored for changes in immunity, virus titers, morbidity, or mortality. Results: In CVB3 infection, mortality was increased in WT males compared to females and males developed more severe cardiac inflammation. Gonadectomy suppressed male, but increased female, susceptibility to CVB3. Infection with IAV resulted in greater morbidity and mortality in WT females compared with males and this sex difference was significantly reduced by gonadectomy of male and female mice. In Gdx FCG mice infected with CVB3, XY mice were less susceptible than XX mice. Protection correlated with increased CD4+ forkhead box P3 (FoxP3)+ T regulatory (Treg) cell activation in these animals. Neither CD4+ interferon (IFN)γ (T helper 1 (Th1)) nor CD4+ interleukin (IL)-4+ (Th2) responses differed among the FCG mice during CVB3 infection. Infection of Gdx FCG mice revealed no effect of sex chromosome complement on morbidity or mortality following IAV infection. Conclusions: These studies indicate that sex chromosome complement can influence pathogenicity of some, but not all, viruses. © 2011 Robinson et al; licensee BioMed Central Ltd.

引用

共 63 条

[61]

Arnold A.P., The organizational-activational hypothesis as the foundation for a unified theory of sexual differentiation of all mammalian tissues, Horm Behav, 55, pp. 570-578, (2009)

[62]

Martin J.T., Sexual dimorphism in immune function: The role of prenatal exposure to androgens and estrogens, Eur J Pharmacol, 405, pp. 251-261, (2000)

[63]

Huber S.A., Kupperman J., Newell M.K., Estradiol prevents and testosterone promotes Fas-dependent apoptosis in CD4+ Th2 cells by altering Bcl 2 expression, Lupus, 8, 5, pp. 384-387, (1999)

← 1 2 3 4 5 6 7 →