In-vitro effect of a combination of 5-fluorouracil (5-FU) and cisplatin (CDDP) on human gastric cancer cell lines: Timing of cisplatin treatment

被引：7

作者：

Cho H. ^{[1
]}

Imada T. ^{[1
]}

Oshima T. ^{[1
]}

Shiozawa M. ^{[1
]}

Rino Y. ^{[1
]}

Takanashi Y. ^{[1
]}

机构：

[1] First Department of Surgery, Yokohama City University, School of Medicine, Yokohama

来源：

Gastric Cancer | 2002年 / 5卷 / 1期

关键词：

5-Fluorouracil; Cisplatin; Gastric cancer;

D O I：

10.1007/s101200200006

中图分类号：

学科分类号：

摘要：

Background. To date, we have few effective chemotherapeutic agents against advanced and recurrent gastric cancer. 5-Fluorouracil (5-FU) and cisplatin (CDDP) are the most widely used drugs, and their combination has demonstrated favorable outcomes. However, the method (especially the timing) of CDDP administration is not well established. Methods. We examined the in-vitro effect of a combination of 5-FU and CDDP on four human gastric cancer cell lines: MKN-1, MKN-28, MKN-45, and MKN-74. The cell lines were exposed to 50% of the inhibitory concentrations of 5-FU and CDDP for 72h and 8h, respectively. CDDP was applied before, simultaneously with, and after the start of treatment with 5-FU. Results. When CDDP was applied after 5-FU, the cytotoxic activity against MKN-28, MKN-45, and MKN-74 was significantly potentiated. Against MKN-1, the earlier the initiation of CDDP treatment, the stronger was the cytotoxic effect. Conclusion. Our results suggest that the cytotoxicity of a combination of 5-FU and CDDP against human gastric cancer cells is both cell-line- and schedule-dependent and is especially affected by the timing of the CDDP treatment.

引用

页码：43 / 46

页数：3

共 14 条

[11]

Kuroki M., Nakano S., Mitsugi K., Ichinose I., Anzai K., Nakamura M., Et al., In vivo comparative therapeutic study of optimal administration of 5-fluorouracil and cisplatin using a newly established HST-1 human squamous-carcinoma cell line, Cancer Chemother Pharmacol, 29, pp. 273-276, (1992)

[12]

Pratesi G., Gianni L., Manzotti C., Zunino F., Sequence dependence of the antitumor and toxic effects of 5-fluorouracil and cis-diamminedichloroplatinum combination on primary colon tumors in mice, Cancer Chemother Pharmacol, 21, pp. 237-240, (1988)

[13]

Imada T., Rino Y., Takahashi M., Amano T., Kusada O., Kitaura K., In vitro combination effect of 5-fluorouracil and cisplatin on the proliferation, morphology and expression of Ki-67 antigen in human gastric cancer cells, Anticancer Drugs, 8, pp. 1000-1006, (1997)

[14]

Kano Y., Akutsu M., Tsunoda S., Suzuki K., Yazawa Y., In vitro schedule-dependent interaction between paclitaxel and cisplatin in human carcinoma cell lines, Cancer Chemother Pharmacol, 37, pp. 525-530, (1996)

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