Evaluation of changes in the activity of proteolytic enzymes and their inhibitors in the processes that accompany the growth of gastric cancer

被引：12

作者：

Kielan W. ^{[1
]}

Suzanowicz J. ^{[1
]}

Siewiński M. ^{[2
]}

Saleh Y. ^{[2
]}

Janocha A. ^{[3
]}

Skalski A. ^{[1
]}

Tarnawa R. ^{[1
]}

机构：

[1] Second Dept. of Gen./Oncol. Surg., Wroclaw Medical University, Wroclaw

[2] First Department of Gynaecology, Wroclaw Medical University, 50-368 Wroclaw

[3] Department of Physiology, Wroclaw Medical University, Wroclaw

来源：

Gastric Cancer | 2004年 / 7卷 / 1期

关键词：

Cathepsin B; Cathepsin L; Cysteine peptidase inhibitors; Cysteine peptidases; Gastric cancer;

D O I：

10.1007/s10120-003-0262-3

中图分类号：

学科分类号：

摘要：

Background. Many investigators have observed a correlation between the aggressiveness of malignant tumor growth and the levels of cysteine peptidases and their antogenous inhibitors. Cathepsins B and L are activated by pepsin in an acidic pH. This fact encouraged us to measure the activity of these enzymes in tissue samples of gastric cancer. Methods. We measured the activities of cathepsins B and L, and their precursors and inhibitors, in homogenates of tissue samples obtained from operations for gastric cancer. We compared the results for the homogenates of tissues from three different sites: the tumor center, the zone of cancer invasion (border of the tumor), and healthy tissue. Results. The highest activities of free cysteine peptidases and those in complexes with their inhibitors, as well as their precursors, were observed in the border of the tumor, while the activities in healthy tissue were significantly lower. Conclusion. The local activities of cysteine peptidases and their inhibitors reflect the topographical differences between the center of the tumor, the zone of invasion, and healthy tissues in gastric cancer patients. In addition, the results for the pattern of changes in the activity of cysteine peptidases according to the degree of tissue infiltration were not dependent on the method of measurement (colorimetry vs spectrofluorometry).

引用

页码：17 / 23

页数：6

共 22 条

[21] Siewinski M., Saleh Y., Popiela A., Ziolkowski P., Jelen M., Grybos M., Expression of high molecular weight cysteine proteinase inhibitor in ovarian cancer tissues: Regulation of cathepsin B expression by placental CPL, Biol Chem, 384, pp. 1103-1107, (2003)
[22] Czecior E., Szymaniec S., Siewinski M., Fortuna W., Miedzybrodzki W., A potential use of cysteine endopeptidases inhibitors of upper respiratory tract tumors, Otolaryngol Pol, 56, pp. 573-576, (2002)

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