Chemiluminescence flow biosensor system for cholesterol with immobilized reagents

被引：17

作者：

Huang Y. ^{[1
]}

Zhang C. ^{[2
]}

Zhang Z. ^{[2
,3
]}

机构：

[1] Department of Chemistry, Nanjing University

[2] Deparlment of Chemistry, Shaanxi Normal University

[3] Department of Chemistry, Southwest Normal University, Beibei

来源：

Analytical Sciences | 1999年 / 15卷 / 9期

关键词：

Chemiluminescence; Cholesterol; Flow sensor; Immobilized reagent;

D O I：

10.2116/analsci.15.867

中图分类号：

学科分类号：

摘要：

A chemiluminescence biosensor combined with flow injection analysis has been developed for determining cholesterol. Cholesterol oxidase was immobilized onto amine-modified silica gel via glutaraldehyde activation and packed in a column. The analytical reagents, including luminol and ferricyanide, were electrostatically coimmobilized on an anion-exchange column. Cholesterol was sensed by the CL reaction between hydrogen peroxide released from the enzymatic reaction and luminol and ferricyanide, which were released from a column with immobilized reagents by elution. The calibration graph was linear over the range 5x10-6 to 1x10-4 g ml-1 and the detection limit was 5x10-6 g ml-1. The proposed method has been successfully applied to the determination of cholesterol in human serum.

引用

页码：867 / 870

页数：3