Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium: Prevention of cell death by AG126 and 15-deoxy-delta 12, 14-PGJ2

被引：75

作者：

Tarun K Garg

Jason Y Chang

机构：

[1] Department of Anatomy, Univ. of Arkansas for Med. Sciences, Little Rock

[2] Department of Ophthalmology, Univ. of Arkansas for Med. Sciences, Little Rock

来源：

BMC Ophthalmology | / 3卷 / 1期

关键词：

Retinal Pigment Epithelium; Retinal Pigment Epithelium Cell; H2O2 Treatment; Photoreceptor Outer Segment; Human Retinal Pigment Epithelium Cell;

D O I：

10.1186/1471-2415-3-5

中图分类号：

学科分类号：

摘要：

Background: The retina, which is exposed to both sunlight and very high levels of oxygen, is exceptionally rich in polyunsaturated fatty acids, which makes it a favorable environment for the generation of reactive oxygen species. The cytotoxic effects of hydrogen peroxide (H2O2) induced)oxidative stress on retinal pigment epithelium were characterized in this study. Methods: The MTT cell viability assay, Texas-Red phalloidin staining, immunohistochemistry and Western blot analysis were used to assess the effects of oxidative stress on primary human retinal pigment epithelial cell cultures and the ARPE-19 cell line. Results: The treatment of retinal pigment epithelial cells with (H2O2 caused a dose-dependent decrease of cellular viability, which was preceded by a significant cytoskeletal rearrangement, activation of the Extracellular signal-Regulated Kinase, lipid peroxidation and nuclear condensation. This cell death was prevented partially by the prostaglandin derivative, 15d-PGJ 2 and by the protein kinase inhibitor, AG126. Conclusion: 15d-PGJ2 and AG126 may be useful pharmacological tools in the future capable of preventing oxidative stress induced RPE cell death in human ocular diseases.

引用

页码：1 / 15

页数：14

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