Development of a nutrient mist bioreactor for growth of hairy roots

被引：28

作者：

Liu C.Z. ^{[1
]}

Wang Y.C. ^{[1
]}

Zhao B. ^{[1
]}

Guo C. ^{[1
]}

Ouyang F. ^{[1
]}

Ye H.C. ^{[2
]}

Li G.F. ^{[2
]}

机构：

[1] Stt. Key Lab. of Biochem. Eng., Institute of Chemical Metallurgy, Chinese Academy of Sciences, P.O. Box 353

[2] Institute of Botany, Chinese Academy of Sciences

来源：

In Vitro Cellular & Developmental Biology - Plant | 1999年 / 35卷 / 3期

关键词：

Artemisia annua L; Growth; Hairy root cultures; Mist bioreactor;

D O I：

10.1007/s11627-999-0091-0

中图分类号：

学科分类号：

摘要：

Hairy root cultures of Artemisia annua L. were cultivated in three different mist bioreactors, each fitted with three stainless steel meshes. The growth rates in the three 2.3-L mist bioreactors differed. After 25 d, the growth index (final dry weight/initial dry weight) of the roots was 42 in a nutrient mist bioreactor, 61 in an inner-loop nutrient mist bioreactor, and 68 in a modified inner-loop nutrient mist bioreactor. Under a misting cycle of 3/30 (ON 3 min/OFF 30 min) for 25 d, dry weight reached 13.6 g/L of medium in the modified inner-loop nutrient mist bioreactor in which nutrient could be supplied without dilution of mist by air flow.

引用

页码：271 / 274

页数：3

共 26 条

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