Embryogenic mango cultures selected for resistance to Colletotrichum gloeosporioides culture filtrate show variation in random amplified polymorphic DNA (RAPD) markers

被引：10

作者：

Jayasankar S. ^{[1
]}

Litz R.E. ^{[1
]}

Schnell R.J. ^{[2
]}

Hernandez A.-C. ^{[3
]}

机构：

[1] Tropical Research and Education Center, University of Florida, Homestead, FL 33031-3314

[2] USDA ARS, Subtropical Horticulture Research Station, Miami, FL 33158

[3] CINVESTAV, Unidad Irapuato, Irapuato, Guanajuato

来源：

In Vitro Cellular & Developmental Biology - Plant | 1998年 / 34卷 / 2期

关键词：

Anthracnose; DNA markers; In vitro selection; Mangifera indica; Somatic embryo;

D O I：

10.1007/BF02822774

中图分类号：

学科分类号：

摘要：

Genomic DNA isolated from embryogenic cultures of two mango cultivars, 'Hindi' and 'Carabao,' that had been selected for resistance to the culture filtrate of Colletotrichum gloeosporioides, was analyzed using Randomly Amplified Polymorphic DNA (RAPD). In vitro selection caused changes in RAPD markers in the selected embryogenic cultures with respect to the unchallenged control cultures and the stock plants. The differences involved both the absence and the presence of additional RAPD markers in the resistant lines, although the former was most commonly observed. The absence of differences between the unchallenged control of either cultivar and DNA from the leaves of parent trees confirmed that the changes were not due to prolonged maintenance in liquid cultures.

引用

页码：112 / 116

页数：4

共 33 条

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