Genetic diversity of sea buckthorn (Hippophae rhamnoides) populations in northeastern and northwestern China as revealed by ISSR markers

被引：3

作者：

Chunjie Tian

Yidong Lei

Suhua Shi

Peng Nan

Jiakuan Chen

Yang Zhong

机构：

[1] Fudan University,Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, and Institute of Biodiversity Science

[2] Zhongshan University,Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences

[3] Shanghai Center for Bioinformation Technology,undefined

来源：

New Forests | 2004年 / 27卷

关键词：

Genetic diversity; ISSR; N; -fixing tree; Sea buckthorn;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

As a N2-fixing tree species, sea buckthorn (Hippophae rhamnoides) is well adapted to arid regions and is utilized for multiple purposes in China. Current knowledge of genetic variability of H. rhamnoides is limited in terms of rangewide distributions. Eleven natural populations of sea buckthorn in northeastern and northwestern China were analyzed to detect genetic variation among and within populations, by use of ISSR (inter-simple sequence repeats) markers. Using eight primers, 207 polymorphic loci were observed, ranging in size from 250 to 2500 bp. The coefficient of gene differentiation (Gst = 0.0679) showed that the total molecular variance of 11 populations was mainly existed within populations. The genetic variation within and among the 11 populations was 93.21 and 6.79%, respectively. No significant correlation between genetic and geographic distances of the populations was found using ISSR markers. Our study provides a population-level genetic profile for further investigation and conservation of genetic diversity of sea buckthorn.

引用

页码：229 / 237

页数：8

共 52 条

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