Cross-species amplification of microsatellite primers in passerine birds

被引：64

作者：

Galbusera P. ^{[1
]}

van Dongen S. ^{[1
]}

Matthysen E. ^{[1
]}

机构：

[1] Laboratory of Animal Ecology, Department of Biology, University of Antwerp (UIA), Wilrijk, B-2610

来源：

Conservation Genetics | 2000年 / 1卷 / 2期

关键词：

Cross-amplification; Microsatellite; Passerine;

D O I：

10.1023/A:1026587024065

中图分类号：

学科分类号：

摘要：

Developing species specific microsatellite primers can be avoided by using existing markers which amplify across species. However, for passerines, such 'cross-species' markers are mostly lacking and few guidelines exist for selecting them from the wide range of existing markers. Here cross-species amplification tests of 40 microsatellite primers in 13 passerine species show an increase in probability of amplification and polymorphism with decreasing phylogenetic distance. Primers which successfully amplified in many species had a higher chance to be polymorphic. However, since the amplification success, across a broad range of species, of particular primersets remains difficult to predict it is crucial to identify such markers empirically. Here we describe such widely applicable bird (passerines) microsatellite markers.

引用

页码：163 / 168

页数：5

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