The culture of chicken embryo fibroblast cells on microcarriers to produce infectious bursal disease virus

被引：7

作者：

Li Zhang

Yuanxing Zhang

Chun Yan

Juntang Yu

机构：

[1] East China University of Science and Technology (ECUST),State Key Laboratory of Bioreactor Engineering

来源：

Applied Biochemistry and Biotechnology | 1997年 / 62卷

关键词：

Bioreactor; chicken embryo fibroblast cell; cell culture; infectious bursal disease virus; microcarrier; plating kinetics;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The cultures of chicken embryo fibroblast (CEF) cells in flasks, spinner bottles, and bioreactors were studied. The growth and metabolism characteristics of CEF cells and the feasibility of the CEF cell culture in bioreactor were investigated. The plating process of the CEF cells on GT-2 microcarriers in spinner bottles was studied, and a plating kinetic model was presented. The culture of CEF cells in 1.5 L CelliGen bioreactor to produce infectious bursal disease virus (IBDV) had met success. Whereas the additive microcarriers were fed during the culture, the cell density was increased 10 times as against seed cells adhering to microcarriers and the virus titer was as high as 7.5. All the aforementioned experimental results have laid the foundation for high density culture of CEF cells and process scale-up.

引用

页码：291 / 302

页数：11