Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis

被引：97

作者：

Randolph B Caldwell

Andrzej M Kierzek

Hiroshi Arakawa

Yuri Bezzubov

Jolanta Zaim

Petra Fiedler

Stefan Kutter

Artem Blagodatski

Diyana Kostovska

Marek Koter

Jiri Plachy

Piero Carninci

Yoshihide Hayashizaki

Jean-Marie Buerstedde

机构：

[1] GSF,Institute of Molecular Radiobiology

[2] Polish Academy of Sciences,Laboratory of Systems Biology Institute of Biochemistry and Biophysics

[3] University of Surrey,School of Biomedical and Molecular Sciences

[4] Institute of Molecular Genetics,Academy of Sciences of the Czech Republic

[5] RIKEN Tsukuba Institute,Laboratory for Genome Exploration Research Project, Genomic Sciences Center and Genome Science Laboratory

[6] RIKEN Yokohama Institute,Genome Exploration Research Group, RIKEN Genomic Sciences Center

来源：

Genome Biology | / 6卷 / 1期

关键词：

Start Codon; cDNA Insert; Translation Start Codon; Alternative Start Codon; Chicken Genome Sequence;

D O I：

10.1186/gb-2004-6-1-r6

中图分类号：

学科分类号：

摘要：

A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential of a large fraction of B-cell specific and housekeeping genes whose function can be analyzed by disruption in the chicken DT40 B-cell line.

引用

共 143 条

[1]

Smithies O(1993)Animal models of human genetic diseases. Trends Genet 9 112-116

[2]

Buerstedde JM(1991)Increased ratio of targeted to random integration after transfection of chicken B cell lines. Cell 67 179-188

[3]

Takeda S(1999)Genetic analysis of B cell antigen receptor signalling. Annu Rev Immunol 17 555-592

[4]

Kurosaki T(2004)Immunoglobulin gene conversion: insights from bursal B cells and the DT40 cell line. Dev Dyn 229 458-464

[5]

Arakawa H(1996)Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability. Genes Dev 10 2588-2599

[6]

Buerstedde JM(1997)Efficient conditional mutation of the vertebrate CENP C gene. Hum Mol Genet 6 2301-2308

[7]

Wang J(2001)Mutant lox vectors for selectable marker recycle and conditional knock-outs. BMC Biotechnol 1 7-5508

[8]

Takagaki Y(1998)Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J 17 5497-716

[9]

Manley JL(2004)Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432 695-573

[10]

Fukagawa T(2002)Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature 420 563-1519

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