Transposon tagging in rice

被引:15
作者
Takeshi Izawa
Tohru Ohnishi
Toshitsugu Nakano
Nobuhiro Ishida
Hiroyuki Enoki
Hisako Hashimoto
Kimiko Itoh
Rie Terada
Chuanyn Wu
Chikara Miyazaki
Tomoko Endo
Shigeru Iida
Ko Shimamoto
机构
[1] Nara Institute of Science and Technology,Laboratory of Plant Molecular Genetics
[2] Plantech Research Institute,Department of Biological Science and Technology
[3] Science University of Tokyo,Department of Agriculture
[4] National Institute for Basic Biology,Nagasaki Laboratory
[5] Niigata University,undefined
[6] National Institute of Agrobiological Resources,undefined
[7] Japan Science and Technology Cooperation,undefined
[8] National Institute of Fruit Tree Science,undefined
来源
Plant Molecular Biology | 1997年 / 35卷
关键词
rice; Ac/Ds; transposon; gene isolation; disease resistance; flowering time;
D O I
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中图分类号
学科分类号
摘要
To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.
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页码:219 / 229
页数:10
相关论文
共 165 条
[1]  
Aarts MGM(1993)Transposon tagging of a male sterility gene in Nature 363 715-717
[2]  
Dirkse WG(1995)/ Mol Gen Genet 247 646-652
[3]  
Stiekema WJ(1987) transposon mutagenesis in EMBO J 6 1547-1554
[4]  
Pereira A(1986): mutant spectrum and frequency of Proc Natl Acad Sci USA 83 4844-4848
[5]  
Altmann T(1993) insertion mutants Genetics 134 1221-1229
[6]  
Felix G(1993)Phenotypic assay for excision of the maize controlling element Mol Gen Genet 240 65-72
[7]  
Jessop A(1993) in tobacco Plant Cell 5 631-638
[8]  
Kauschmann A(1984)Transposition of the maize controlling element Mol Gen Genet 194 346-347
[9]  
Uwer U(1996) in tobacco Plant J 9 935-945
[10]  
Pena-Cortes H(1991)Transposition pattern of the maize element Mol Gen Genet 229 365-372