Transposon tagging in rice

被引:15
作者
Takeshi Izawa
Tohru Ohnishi
Toshitsugu Nakano
Nobuhiro Ishida
Hiroyuki Enoki
Hisako Hashimoto
Kimiko Itoh
Rie Terada
Chuanyn Wu
Chikara Miyazaki
Tomoko Endo
Shigeru Iida
Ko Shimamoto
机构
[1] Nara Institute of Science and Technology,Laboratory of Plant Molecular Genetics
[2] Plantech Research Institute,Department of Biological Science and Technology
[3] Science University of Tokyo,Department of Agriculture
[4] National Institute for Basic Biology,Nagasaki Laboratory
[5] Niigata University,undefined
[6] National Institute of Agrobiological Resources,undefined
[7] Japan Science and Technology Cooperation,undefined
[8] National Institute of Fruit Tree Science,undefined
来源
Plant Molecular Biology | 1997年 / 35卷
关键词
rice; Ac/Ds; transposon; gene isolation; disease resistance; flowering time;
D O I
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中图分类号
学科分类号
摘要
To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.
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页码:219 / 229
页数:10
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