Genome-wide comparative analysis of microRNAs in three non-human primates

被引：27

作者：

Brameier M. ^{[1
]}

机构：

[1] Primate Genetics Laboratory, German Primate Center, Leibniz Institute for Primate Research

来源：

BMC Research Notes | / 3卷 / 1期

关键词：

miRNA Precursor; Mature Sequence; Human miRNAs; Negative Prediction; Precursor Sequence;

D O I：

10.1186/1756-0500-3-64

中图分类号：

学科分类号：

摘要：

Background. MicroRNAs (miRNAs) are negative regulators of gene expression in multicellular eukaryotes. With the recently completed sequencing of three primate genomes, the study of miRNA evolution within the primate lineage has only begun and may be expected to provide the genetic and molecular explanations for many phenotypic differences between human and non-human primates. Findings. We scanned all three genomes of non-human primates, including chimpanzee (Pan troglodytes), orangutan (Pongo pygmaeus), and rhesus monkey (Macaca mulatta), for homologs of human miRNA genes. Besides sequence homology analysis, our comparative method relies on various postprocessing filters to verify other features of miRNAs, including, in particular, their precursor structure or their occurrence (prediction) in other primate genomes. Our study allows direct comparisons between the different species in terms of their miRNA repertoire, their evolutionary distance to human, the effects of filters, as well as the identification of common and species-specific miRNAs in the primate lineage. More than 500 novel putative miRNA genes have been discovered in orangutan that show at least 85 percent identity in precursor sequence. Only about 40 percent are found to be 100 percent identical with their human ortholog. Conclusion. Homologs of human precursor miRNAs with perfect or near-perfect sequence identity may be considered to be likely functional in other primates. The computational identification of homologs with less similar sequence, instead, requires further evidence to be provided. © 2010 Brameier; licensee BioMed Central Ltd.

引用

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