Morphohistological study of the different constituents of a banana (Musa AAA, cv. Grande naine) embryogenic cell suspension

被引：34

作者：

Georget F. ^{[1
,2
]}

Domergue R. ^{[1
]}

Ferrière N. ^{[1
]}

Côte F.X. ^{[1
]}

机构：

[1] Ctr. Coop. Internationale en Rech., Agronomique pour le Developpement, CIRAD-Flhor and CIRAD-Biotrop, F-34032 Montpellier Cedex 01

[2] Vitropic S.A. Z.A.E. Les Avants L.

来源：

Plant Cell Reports | 2000年 / 19卷 / 8期

关键词：

Banana; Cell suspension; Histology; Somatic embryogenesis;

D O I：

10.1007/s002999900188

中图分类号：

学科分类号：

摘要：

Five types of cellular aggregates have been characterised in embryogenic cell suspensions of banana (Musa AAA Grande naine cv.). Type I corresponded to isolated cells or to small cell aggregates. Type II were composed of embryogenic cells. Type III can be distinguished from type II due to the presence of peripheral proliferation zones with embryonic cells. Type IV were composed of protodermic masses histologically comparable to proembryos. Type V were nodules composed of a central zone of meristematic cells and of an external zone of starchy cells. Each culture flask of a cell line contained a majority of one of the above-mentioned aggregate types. Histological studies of somatic embryo development on semi-solid regeneration medium showed that there were close similarities between the initial steps of ontogenesis of the embryos and the different cell aggregates in liquid multiplication medium. It appeared that aggregates II-IV of the suspension belong to the same development continuum which reproduces the initial phases of somatic embryo ontogenesis on semi-solid medium. Type V resulted from the development of type IV, for which ontogenesis is hindered by direct contact with 2.4-dichlorophenoxyacetic acid and the shaken liquid multiplication medium. Type I aggregates probably do not belong to the development continuum but rather correspond to the degeneration of the other types of aggregates in the suspension. The presence of intermediate types in the liquid medium reinforces the hypothesis of a relationship between the aggregates. The aggregates tended to develop through time from a majority of type II or III at the beginning of their culture to types IV-V for older suspensions.

引用

页码：748 / 754

页数：6

共 26 条

[1]

Cote F.X., Domergue R., Monmarson S., Schwendiman J., Teisson C., Escalant J.V., Embryogenic cell suspensions from the male flower of Musa AAA cv. Grand nain, Physiol Plant, 97, pp. 285-290, (1996)

[2]

Cronauer S.S., Krikorian A.D., Plant regeneration via somatic embryogenesis in the seeded diploid banana Musa ornate Roxb, Plant Cell Rep, 7, pp. 23-25, (1988)

[3]

De Touchet B., Duval Y., Pannetier C., Plant regeneration from embryogenic suspension cultures of oil palm (Elaeis guineensis Jacq.), Plant Cell Rep, 10, pp. 529-532, (1991)

[4]

Dhed'a D., Dumortier F., Panis B., Vuylsteke D., De Langhe E., Plant regeneration in cell suspension cultures of cooking banana "Bluggoe" cultivar (Musa spp. ABB group), Fruits, 46, pp. 125-135, (1991)

[5]

Durham R.E., Parott W.A., Repetitive somatic embryogenesis from peanut cultures in liquid medium, Plant Cell Rep, 11, pp. 122-125, (1992)

[6]

Emons A.M.C., Kieft H., Histological comparison of single somatic embryos of maize from suspension culture with somatic embryos attached to callus cells, Plant Cell Rep, 10, pp. 485-488, (1991)

[7]

Escalant J.V., Teisson C., Somatic embryogenesis and plants from immature zygotic embryos of species Musa acuminata and Musa balbisiana, Plant Cell Rep, 7, pp. 181-186, (1989)

[8]

Finner J.J., Nagasawa A., Development of an embryogenic suspension culture of soybean (Glycine max Merill.), Plant Cell Rep, 11, pp. 323-328, (1988)

[9]

Fisher D.B., Protein staining of ribboned epon sections for light microscopy, Histochemie, 16, pp. 92-96, (1968)

[10]

Gavish H., Vardi A., Fluhr R., Extracellular proteins and early embryo development in in Citrus nucellar cell cultures, Physiol Plant, 82, pp. 606-616, (1991)

← 1 2 3 →