Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1α by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats

被引：36

作者：

Hofmann N. ^{[1
]}

Lachnit N. ^{[1
]}

Streppel M. ^{[2
]}

Witter B. ^{[1
]}

Neiss W. ^{[1
]}

Guntinas-Lichius O. ^{[2
]}

Angelov D.N. ^{[1
]}

机构：

[1] Institut Anatomie Universitat Koln, Köln

[2] Klinik fur Hals-Nasen/Ohrenheilkunde, Universität zu Köln, Köln

来源：

BMC Immunology | / 3卷 / 1期

关键词：

Major Histocompatibility Complex Class; Myelin Basic Protein; Experimental Allergic Encephalomyelitis; Black Reaction; Perivascular Macrophage;

D O I：

10.1186/1471-2172-3-11

中图分类号：

学科分类号：

摘要：

Background: T-cells extravasation and CNS parenchyma infiltration during autoimmune neurodegenerative disease can be evoked by local antigen presenting cells. Studying the chemoattracting potential of spinal perivascular macrophages (SPM) during experimental allergic encephalomyelitis (EAE), we observed numerous infiltrates of densely-packed mononuclear cells. Apart from the poor spatial and optical resolution, no differentiation between the resident SPM (mabs ED1+, ED2+) and the just recruited monocytes/macrophages (mab ED1+) was possible. Results: This is why we labeled SPM by injections of different fluoresecent dyes into the lateral cerebral ventricle before induction of active EAE. Within an additional experimental set EAE was induced by an intraperitoneal injection of T-cells specifically sensitized to myelin basic protein (MBP) and engineered to express the green fluorescent protein (GFP). In both experiments we observed a strong activation of SPM (mabs OX6+, SILK6+, CD40+, CD80+, CD86+) which was accompanied by a consistently increased expression of ICAM-1, VCAM-1, and the chemokines MCP-1 and MIP-1α. Conclusion: These observations indicate that SPM play a role in promoting lymphocyte extravasation. © 2002 Hofmann et al; licensee BioMed Central Ltd.

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