Expression of the farnesyldiphosphate synthase gene of Saccharomyces cerevisiae in tobacco

被引：1

作者：

S. Daudonnet

F. Karst

Y. Tourte

机构：

[1] Institut de Biologie Moléculaire de d'Ingénierie Génétique,Laboratoire de Biologie Cellulaire Végétale et

[2] Institut de Biologie Moléculaire de d'Ingénierie Génétique,Laboratoire de Génétique Physiologique et Moléculaire

[3] Verneuil Recherche,undefined

[4] Ferme de l'Étang,undefined

来源：

Molecular Breeding | 1997年 / 3卷

关键词：

farnesyldiphosphate synthase expression; tobacco; sterol synthesis; carotenoid synthesis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The coding region of the farnesyldiphosphate synthase (FDP synthase) gene from Saccharomyces cerevisiae has been inserted into a pBin19 vector, downstream of the cauliflower mosaic virus (CaMV) 35S promoter, in order to allow its expression in the genome of a higher plant, Nicotiana tabacum. We have produced transgenic tobacco in which the expression of the foreign gene leads to functional FDP synthase activity. In these transgenic plants, total FDP synthase-specific activity is increased 12-fold compared to controls. This increase of FDP synthase activity has been correlated to a clear increase of both sterol and carotenoid biosynthesis. This heterologous expression is also related to an increased resistance of transformed plants to R172117, a specific inhibitor of FDP synthase, and to sterol biosynthesis inhibitors such as flusilazol and fenpropimorph.

引用

页码：137 / 145

页数：8

共 112 条

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