Ultrafast enzymatic reaction dynamics in protochlorophyllide oxidoreductase

被引：62

作者：

Derren J Heyes

C Neil Hunter

Ivo H M van Stokkum

Rienk van Grondelle

Marie Louise Groot

机构：

[1] Department of Molecular Biology, University of Sheffield

[2] Department of Exact Sciences, Vrije Universiteit

来源：

Nature Structural & Molecular Biology | 2003年 / 10卷 / 6期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1038/nsb929

中图分类号：

学科分类号：

摘要：

The reaction catalyzed by the light-driven enzyme protochlorophyllide oxidoreductase (POR) has been initiated with a 50-fs laser pulse. We show that the catalytic mechanism, involving proton and hydride transfers, proceeds with time constants of 3 ps and 400 ps. It is known that catalysis by POR involves thermally excited protein dynamics; our results show that these molecular motions occur on an ultrafast timescale.

引用

页码：491 / 492

页数：1

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