Soluble carbohydrate content of soybean [Glycine max (L.) Merr.] Somatic and zygotic embryos during development

被引：23

作者：

Chanprame S. ^{[1
]}

Kuo T.M. ^{[2
]}

Widholm J.M. ^{[1
]}

机构：

[1] University of Illinois, Department of Crop Sciences, Edward R. Madigan Laboratory, Urbana, IL 61801

[2] National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL 61604

来源：

In Vitro Cellular & Developmental Biology - Plant | 1998年 / 34卷 / 1期

关键词：

Oil; Protein; Soluble carbohydrate; Somatic embryo; Soybean; Zygotic embryos;

D O I：

10.1007/BF02823125

中图分类号：

学科分类号：

摘要：

Somatic and zygotic embryos of soybean cv. Jack were analyzed for soluble carbohydrate, total lipids, and protein during development. Zygotic embryos accumulated trace amounts of fructose, galactose, and galactinol, whereas somatic embryos contained only trace amounts of galactose. Somatic embryos accumulated much higher glucose levels than zygotic embryos. Both somatic and zygotic embryos contain low levels of sucrose, myoinositol, and pinitol. Raffinose and stachyose accumulated in the late developmental stages of zygotic embryos, but only stachyose was found to accumulate in the late stage somatic embryos. Zygotic embryos contained low total lipid levels up to 50 d after flowering (DAF) and then the levels increased to 16% by 55 DAF and 21% at 65 DAF. Somatic embryos had low levels of total lipids throughout development with the maximum of only 4.7%. Soybean zygotic embryos contained about 40% protein throughout development, while the protein concentration of somatic embryos decreased from 44% to 25% as maturation approached. These studies demonstrate that the composition of Jack zygotic embryos is similar to that described for other cultivars during development while the somatic embryo composition and size is markedly different. The low somatic embryo germination often noted might be due to the abnormal development as shown by a composition different from that of mature zygotic embryos. The low concentration of the raffinose series sugars might be especially important factors.

引用

页码：64 / 68

页数：4

共 39 条

[11] Finer J.J., Nagasawa A., Development of an embryogenic suspension culture of soybean, Plant Cell Tissue Organ Cult., 15, pp. 125-136, (1988)
[12] Finer J.J., Vain P., Jones M.W., Et al., Development of the particle inflow gun for DNA delivery to plant cells, Plant Cell Rep., 11, pp. 323-328, (1992)
[13] Gamborg O.L., Miller R.A., Ojima K., Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res., 50, pp. 150-158, (1968)
[14] Gascon S., Lampen J.O., Purification of the internal invertase of yeast, J. Biol. Chem., 243, pp. 1567-1572, (1968)
[15] Hartweck L.M., Lazzeri P.A., Cui D., Et al., Auxin-orientation effects on somatic embryogenesis from immature soybean cotyledons, In Vitro Cell. Dev. Biol., 24, pp. 821-828, (1988)
[16] Hill J.E., Breidenbach R.W., Proteins of soybean seeds I. Isolation and characterization of major components, Plant Physiol., 53, pp. 742-746, (1974)
[17] Hill J.E., Breidenbach R.W., Proteins of soybean seeds II. Accumulation of the major protein components during seed development and maturation, Plant Physiol., 53, pp. 747-751, (1974)
[18] Horbowicz M., Obendorf R.L., McKersie B.D., Et al., Soluble saccharides and cyclitols in alfalfa (Medicago sativa L.) somatic embryos, leaflets and mature seeds, Plant Sci., 109, pp. 191-198, (1995)
[19] Hymowitz T., Collins F.I., Panczner J., Et al., Relationship between the content of oil, protein and sugar in soybean seeds, Agron. J., 64, pp. 613-616, (1972)
[20] Hymowitz T., Walker W.M., Collins F.I., Et al., Stability of sugar content in soybean strains, Commun. Soil Sci. Plant Anal., 3, pp. 367-373, (1972)

← 1 2 3 4 →