Tween-20 activates and solubilizes the mitochondrial membrane-bound, calmodulin dependent NAD+ kinase of Avena sativa L.

被引:20
作者
Pou de Crescenzo M.-A. [1 ]
Gallais S. [1 ]
Léon A. [1 ]
Laval-Martin D.L. [1 ]
机构
[1] Equipe de Biochimie et de Biologie Moléculaire, UFR Sciences, Université d'Angers, 49 045 Angers Cedex 01
关键词
Calmodulin-dependent NAD[!sup]+[!/sup] kinase; K[!sub]m[!/sub; Mitochondrial membranes; Solubilization; Tween-20; V[!sub]max[!/sub;
D O I
10.1007/s0023201-0039-8
中图分类号
学科分类号
摘要
Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 M NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD+ kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD+ kinase could occur in vivo in Avena sativa and could generate a soluble isoform.
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页码:135 / 146
页数:11
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