Tween-20 activates and solubilizes the mitochondrial membrane-bound, calmodulin dependent NAD+ kinase of Avena sativa L.

被引：20

作者：

Pou de Crescenzo M.-A. ^{[1
]}

Gallais S. ^{[1
]}

Léon A. ^{[1
]}

Laval-Martin D.L. ^{[1
]}

机构：

[1] Equipe de Biochimie et de Biologie Moléculaire, UFR Sciences, Université d'Angers, 49 045 Angers Cedex 01

来源：

The Journal of Membrane Biology | 2001年 / 182卷 / 2期

关键词：

Calmodulin-dependent NAD[!sup]+[!/sup] kinase; K[!sub]m[!/sub; Mitochondrial membranes; Solubilization; Tween-20; V[!sub]max[!/sub;

D O I：

10.1007/s0023201-0039-8

中图分类号：

学科分类号：

摘要：

Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 M NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD+ kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD+ kinase could occur in vivo in Avena sativa and could generate a soluble isoform.

引用

页码：135 / 146

页数：11

共 48 条

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