Direct determination of unbound lipophilic ligands in aqueous solutions

被引：2

作者：

Bojesen I.N. ^{[1
]}

机构：

[1] Department of Medical Biochemistry and Genetics, University of Copenhagen, The Panum Institute, DK-2200 Copenhagen N

来源：

Biological Procedures Online | 2004年 / 6卷 / 1期

关键词：

Binding sites; Erythrocyte membrane; Ligands;

D O I：

10.1251/bpo93

中图分类号：

学科分类号：

摘要：

Due to their hydrophobic nature, lipophilic compounds are always bound to proteins when transported in the organism. The transfer of such compounds between their binding proteins and cells as well as intracellular trafficking is mediated by a very low water-phase concentration of monomers. The use of protein filled resealed red cell membranes (erythrocyte ghosts) as semipermeable bags enables us to determine directly such water-phase concentrations in a biological system where the lipophilic compound is in equilibrium with the compound bound to its binding protein. Equilibrium dissociation constants (Kd's) and number of binding sites are determined by regression analyses of data. We describe the method with the hydrophobic anion arachidonate and the neutral N-arachidonoylethanolamide as examples. © 2004 by the author(s).

引用

页码：226 / 234

页数：8

共 27 条

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