Structure of α-latrotoxin oligomers reveals that divalent cation- dependent tetramers form membrane pores

被引：114

作者：

Orlova E.V. ^{[1
]}

Atiqur Rahman M. ^{[1
]}

Gowen B. ^{[1
]}

Volynski K.E. ^{[1
]}

Ashton A.C. ^{[1
]}

Manser C. ^{[1
]}

Van Heel M. ^{[1
]}

Ushkaryov Y.A. ^{[1
]}

机构：

[1] Biochemistry Department, Imperial College, London SW7 2AY, Exhibition Road

来源：

Nature Structural Biology | 2000年 / 7卷 / 1期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1038/71247

中图分类号：

学科分类号：

摘要：

We report here the first three-dimensional structure of α-latrotoxin, a black widow spider neurotoxin, which forms membrane pores and stimulates secretion in the presence of divalent cations. We discovered that α- latrotoxin exists in two oligomeric forms: it is dimeric in EDTA but forms tetramers in the presence of Ca2+ or Mg2+. The dimer and tetramer structures were determined independently at 18 Å and 14 Å resolution, respectively, using cryo-electron microscopy and angular reconstitution. The α-latrotoxin monomer consists of three domains. The N- and C-terminal domains have been identified using antibodies and atomic fitting. The CA- symmetric tetramers represent the active form of α-latrotoxin; they have an axial channel and can insert into lipid bilayers with their hydrophobic base, providing the first model of α-latrotoxin pore formation.

引用

页码：48 / 53

页数：5

共 34 条

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