Promoter trapping in Magnaporthe grisea.

被引:4
作者
Liu X.H. [1 ]
Lu J.P. [1 ]
Wang J.Y. [1 ]
Min H. [1 ]
Lin F.C. [1 ]
机构
[1] Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou
来源
Journal of Zhejiang University SCIENCE B | 2006年 / 7卷 / 1期
基金
中国国家自然科学基金;
关键词
Promoter trapping; Green fluorescent protein; Magnaporthe grisea; A; Q949.32;
D O I
10.1631/jzus.2006.B0028
中图分类号
学科分类号
摘要
Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1,077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.
引用
收藏
页码:28 / 33
页数:5
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