Promoter trapping in Magnaporthe grisea.

被引：4

作者：

Liu X.H. ^{[1
]}

Lu J.P. ^{[1
]}

Wang J.Y. ^{[1
]}

Min H. ^{[1
]}

Lin F.C. ^{[1
]}

机构：

[1] Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou

来源：

Journal of Zhejiang University SCIENCE B | 2006年 / 7卷 / 1期

基金：

中国国家自然科学基金;

关键词：

Promoter trapping; Green fluorescent protein; Magnaporthe grisea; A; Q949.32;

D O I：

10.1631/jzus.2006.B0028

中图分类号：

学科分类号：

摘要：

Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1,077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

引用

页码：28 / 33

页数：5

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