数字PCR技术在国门生物安全保障领域的应用

被引：2

作者：

王艺凯 ^{[1
]}

齐玮 ^{[1
]}

杨诣 ^{[2
]}

王占坤 ^{[3
]}

王胜利 ^{[3
]}

陈芊如 ^{[4
]}

孔维恒 ^{[1
]}

芮孝 ^{[3
]}

邱烨 ^{[1
]}

刘鑫 ^{[1
]}

机构：

[1] 中国海关科学技术研究中心

[2] 南佛罗里达大学

[3] 新羿生物科技(北京)有限公司

[4] 清华大学医学院生物医学工程系

来源：

分析仪器 | 2023年 / 06期

关键词：

数字PCR; 高灵敏度; 绝对定量; 国门生物安全;

D O I：

暂无

中图分类号：

Q503 [生物化学技术];

学科分类号：

摘要：

数字PCR作为第三代PCR检测技术，无需校准物和绘制标准曲线即可实现对样品初始浓度的绝对定量，具有高灵敏度、高特异性和高精确度的特点；已被广泛应用于遗传病、癌症、传染病的诊断和拷贝数变异、突变检测、转基因检测等方面的研究。本文对数字PCR技术的发展、原理、数据分析做了介绍，并对数字PCR技术在国门生物安全领域的研究进展和应用前景做了介绍和论述。

引用

页码：109 / 116

页数：8

共 55 条

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