SPECIFIC INTERACTION OF THE NEW FLUORESCENT DYE 10-N-NONYL ACRIDINE-ORANGE WITH INNER MITOCHONDRIAL-MEMBRANE - A LIPID-MEDIATED INHIBITION OF OXIDATIVE-PHOSPHORYLATION

被引：56

作者：

MAFTAH, A ^{[1
]}

PETIT, JM ^{[1
]}

JULIEN, R ^{[1
]}

机构：

[1] FAC SCI LIMOGES, GENIUS UNITE BIOTECHNOL, 123 AVE ALBERT THOMAS, F-87060 LIMOGES, FRANCE

来源：

FEBS LETTERS | 1990年 / 260卷 / 02期

关键词：

(Rat liver mitochondria); ATP synthesis; Nonyl acridine orange; Respiratory chain; mitochondria;

D O I：

10.1016/0014-5793(90)80112-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fluorescent dye 10-N-nonyl acridine orange (NAO), known as specifically associated with mitochondria, has been reported to have a cytotoxic effect when high doses were applied to cells. Presently, the biochemical basis of its toxicity was investigated on isolated rat liver mitochondria. At low concentrations, NAO strongly inhibited state 3 respiration and ATP synthesis. At high concentrations, electron transport, ATP hydrolysis, Pi-transport and adenine nucleotide activities were also decreased. All these inhibitions can be explained by probe-cardiolipin interactions which could induce the collapse of energy conversion and/or the modification of membrane fluidity. © 1990.

引用

页码：236 / 240

页数：5

共 29 条

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