SCANNING TUNNELING MICROSCOPY IMAGING OF TORPEDO ACETYLCHOLINE-RECEPTOR

被引：13

作者：

BERTAZZON, A ^{[1
]}

CONTITRONCONI, BM ^{[1
]}

RAFTERY, MA ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT BIOCHEM,1479 GORTNER AVE,ST PAUL,MN 55108

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 20期

关键词：

SYNAPTIC MEMBRANE; SURFACE DIMENSIONS; CENTRAL CAVITY;

D O I：

10.1073/pnas.89.20.9632

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The synaptic surface of the acetylcholine receptor in membranes from Torpedo californica electric organ has been imaged by scanning tunneling microscopy. The molecule appears pentameric, with one major and four minor protrusions rising above the surface, and these protrusions encompass a large central cavity. The outer diameter of the molecule is 69 +/- 10 angstrom, while the diameter of the cavity, measured at the widest complete contour line delimiting the opening, is 26 +/- 7 angstrom. The images and dimensions obtained are consistent with the structure determined from hybrid density maps obtained by x-ray diffraction and electron microscopy. Thus, scanning tunneling microscopy can be used to obtain overall dimensions and low-resolution structural features of the surface of a membrane-embedded protein.

引用

页码：9632 / 9636

页数：5

共 30 条

[11] FRACTIONATION AND PARTIAL CHARACTERIZATION OF MEMBRANE PARTICLES FROM TORPEDO-CALIFORNICA ELECTROPLAX [J].

DUGUID, JR ;

RAFTERY, MA .

BIOCHEMISTRY, 1973, 12 (19) :3593-3597

[12] DIRECT OBSERVATION OF PHOSPHORYLASE-KINASE AND PHOSPHORYLASE-B BY SCANNING TUNNELING MICROSCOPY [J].

EDSTROM, RD ;

MEINKE, MH ;

YANG, XR ;

YANG, R ;

EVANS, DF .

BIOCHEMISTRY, 1989, 28 (12) :4939-4942

[13] PURIFICATION OF TORPEDO-CALIFORNICA POST-SYNAPTIC MEMBRANES AND FRACTIONATION OF THEIR CONSTITUENT PROTEINS [J].

ELLIOTT, J ;

BLANCHARD, SG ;

WU, W ;

MILLER, J ;

STRADER, CD ;

HARTIG, P ;

MOORE, HP ;

RACS, J ;

RAFTERY, MA .

BIOCHEMICAL JOURNAL, 1980, 185 (03) :667-677

[14] SPECIFIC BINDING OF PERHYDROHISTRIONICOTOXIN TO TORPEDO ACETYLCHOLINE-RECEPTOR [J].

ELLIOTT, J ;

DUNN, SMJ ;

BLANCHARD, SG ;

RAFTERY, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (06) :2576-2579

[15] IMAGING OF UNCOATED PURPLE MEMBRANE BY SCANNING TUNNELING MICROSCOPY [J].

GUCKENBERGER, R ;

HACKER, B ;

HARTMANN, T ;

SCHEYBANI, T ;

WANG, Z ;

WIEGRABE, W ;

BAUMEISTER, W .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (02) :1227-1230

[16]

HORBER JKH, 1990, ABSTRACTS STM 90

[17] IMMUNOSPECIFIC IDENTIFICATION AND 3-DIMENSIONAL STRUCTURE OF A MEMBRANE-BOUND ACETYLCHOLINE-RECEPTOR FROM TORPEDO-CALIFORNICA [J].

KLYMKOWSKY, MW ;

STROUD, RM .

JOURNAL OF MOLECULAR BIOLOGY, 1979, 128 (03) :319-334

[18] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[19]

LINDSAY SM, 1989, SCIENCE, V244, P1062

[20]

MIELKE DK, 1988, J BIOL CHEM, V26, P3177

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