LOCATION OF HEME IN BACTERIOFERRITIN OF ESCHERICHIA-COLI

被引：13

作者：

FROLOW, F ^{[1
]}

KALB, AJ ^{[1
]}

YARIV, J ^{[1
]}

机构：

[1] UNIV BORDEAUX 1, CRISTALLOG & PHYS CRISTALLINE LAB, F-33405 TALENCE, FRANCE

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1993年 / 49卷

关键词：

D O I：

10.1107/S0907444993007073

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A low-resolution partial structure of bacterioferritin was solved using a combination of molecular replacement and rigid-body refinement methods. Modification of bacterioferritin crystals by soaking in tetrachloroplatinate results in a phase transition from tetragonal symmetry (space group P4(2)2(1)2) to a pseudocubic one (approximate space group I432). Helical parts of human H ferritin structure stripped of side chains beyond the c(beta) atoms were used as the model. An electron-density map of the refined model revealed a region of extended density which by its shape and position in a pocket between helices was identified as haem. Inclusion of haem in the refinement showed that it can occupy only one of two symmetry-related sites near a twofold axis of the molecule.

引用

页码：597 / 600

页数：4

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