DIFFERENTIAL GENE-EXPRESSION IN THE MURINE THYMUS ASSAYED BY QUANTITATIVE HYBRIDIZATION OF ARRAYED CDNA CLONES

被引：167

作者：

NGUYEN, C ^{[1
]}

ROCHA, D ^{[1
]}

GRANJEAUD, S ^{[1
]}

BALDIT, M ^{[1
]}

BERNARD, K ^{[1
]}

NAQUET, P ^{[1
]}

JORDAN, BR ^{[1
]}

机构：

[1] CNRS MARSEILLE LUMINY,INSERM,CTR IMMUNOL,F-13288 MARSEILLE 9,FRANCE

来源：

GENOMICS | 1995年 / 29卷 / 01期

关键词：

D O I：

10.1006/geno.1995.1233

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

High-throughput measurement of hybridization signatures obtained using complex probes prepared from poly(A)(+) RNA and high-density cDNA colony filters is described. The performance of the system, elimination of artifacts, and verification of the validity of the data are discussed. cDNAs corresponding to sequences present at levels of approximately 0.01% in the complex probe can be detected. Good correlation is observed between expression profiles determined by this method and by Northern blotting. The method is applied to a preliminary investigation of differential expression in three cell types present in the murine thymus. (C) 1995 Academic Press, Inc.

引用

页码：207 / 216

页数：10

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