GLUTATHIONE TRANSFERASE-PI - ITS MINIMAL PROMOTER AND DOWNSTREAM CIS-ACTING ELEMENT

被引：37

作者：

XIA, CL

COWELL, IG

DIXON, KH

PEMBLE, SE

KETTERER, B

TAYLOR, JB

机构：

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90914-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fragments of the human glutathione S-transferase π gene and 15 kb of its 5′ flanking region have been fused to the chloramphenicol acetyl transferase (CAT) reporter gene. Transfection into a number of human cell lines (Hela, HepG2, MCF7 and EJ) has demonstrated that the AP1 binding site, located between nucleotides -58 and -65 (Cowell et al. 1988, Biochem. J. 255, 79-83), is essential for basal level promoter activity. We have also identified a positive cis-acting DNA element between nucleotides +8 and +72 which seems to be part of the promoter. No other regulatory activity was identified within the 17 kb analyzed. © 1991.

引用

页码：233 / 240

页数：8

共 18 条

[11] THETA, A NEW CLASS OF GLUTATHIONE TRANSFERASES PURIFIED FROM RAT AND MAN [J].