DETERMINATION OF BETAMETHASONE AND DEXAMETHASONE BY DERIVATIZATION AND LIQUID-CHROMATOGRAPHY

被引：11

作者：

WU, SM

CHEN, SH

WU, HL

机构：

[1] Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College, Kaohsiung

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 268卷 / 02期

关键词：

LIQUID CHROMATOGRAPHY; SAMPLE PREPARATION; BETAMETHASONE; DEXAMETHASONE; PHARMACEUTICALS;

D O I：

10.1016/0003-2670(92)85219-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A derivatization method followed by liquid chromatography (LC) is described for the determination of the glucocorticoid epimers of betamethasone and dexamethasone. The method is based on the derivatization of the epimers with 1-ethoxy-4-(dichloro-s-triazinyl)naphthalene in acetonitrile, using potassium carbonate as a base catalyst. The resulting derivatives were readily separated by normal-phase LC. Several parameters that affect the derivatization are discussed. The method is feasible for the determination of betamethasone and dexamethasone in tablets.

引用

页码：255 / 260

页数：6

共 10 条

[1] 1-ETHOXY-4-(DICHLORO-S-TRIAZINYL)NAPHTHALENE - FLUORESCENT REAGENT FOR CORTICOSTEROIDS [J].