MEDIA USED IN THE DETECTION AND ENUMERATION OF STAPHYLOCOCCUS-AUREUS

被引：54

作者：

BAIRD, RM ^{[1
]}

LEE, WH ^{[1
]}

机构：

[1] US FOOD SAFETY & INSPECT SERV,BELTSVILLE,MD

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1995年 / 26卷 / 01期

关键词：

STAPHYLOCOCCUS AUREUS; FOOD POISONING; SELECTIVE MEDIA; ENRICHMENT MEDIA; DIAGNOSTIC MEDIA;

D O I：

10.1016/0168-1605(93)E0028-P

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Over the years a range of selective and diagnostic media have been developed to assist in the detection and enumeration of Staphylococcus aureus in routine food surveillance programmes and food poisoning investigations and these are reviewed here. Baird-Parker agar remains, however, the medium of choice for direct plating and enumeration of S. aureus in both Europe and the US. This paper also reports on a comparison of the productivity and selectivity of trypticase soy broth with 10% NaCl and 1% sodium pyruvate (PTSBS) with trypticase soy broth (TSB) for the isolation of S. aureus. Using three strains of S. aureus and a strain of S. hyicus the productivity ratio of PTSBS to TSBS ranged from -0.17 to 0.57. In the recovery of heat-injured cells of S. aureus, PTSBS offered little or no improvement over TSB with sodium chloride but no pyruvate in a three-tube MPN assessment. Of the seven other bacterial cultures tested, none grew well on the PTSBS. Selectivity ratios of 4.4-7 were found.

引用

页码：15 / 24

页数：10

共 48 条

[31] Lancette, Current resuscitation methods for recovery of stressed Staphylococcus aureus from foods, J. Food Protect., 49, pp. 477-481, (1986)
[32] Lancette, Lanier, Most probable number method for isolation and enumeration of Staphylococcus aureus in food: collaborative study, J. Assoc. Off. Anal. Chem., 70, (1987)
[33] Mintzer-Morgenstern, Katzenelson, A simple medium for the isolation of coagulase-positive staphylococci in a single step, J. Food Protect., 45, pp. 218-222, (1982)
[34] Modi, Rose, Trantner, The effect of irradiation and temperature on the immunological activity of staphylococcal enterotoxin A, Int. Food Microbiol., 11, pp. 85-92, (1990)
[35] Mossel, Elderink, A simple test for the assessment of the suitability of pork plasma for incorporation into Baird-Parker base medium for the enumeration of Staphylococcus aureus in foods and other specimens, Lab. Pract., 28, (1979)
[36] Orth, Anderson, Polymyxin-coagulase mannitol agar. 1. A selective isolation medium for coagulase positive staphylococci, Appl. Microbiol., 19, pp. 73-75, (1970)
[37] Ray, Current methods to detect stressed microorganisms, J. Food Protect., 42, pp. 346-355, (1979)
[38] Sawhney, The toxicity of potassium tellurite to Staphylococcus aureus in rabbit plasma fibrinogen agar, J. Appl. Bacteriol., 61, pp. 149-155, (1986)
[39] Schmitt, Schuler-Schmid, Schmidt-Lorenze, Temperature limits of growth, TNase and enterotoxin production of Staphylococcus aureus strains isolated from foods, Int. J. Food Microbiol., 11, pp. 1-20, (1990)
[40] Schwabe, Notermans, Boot, Tatini, Kramer, Inactivation of staphylococcus enterotoxins by heat and reactivation by high pH treatment, Int. J. Food Microbiol., 10, pp. 33-42, (1990)

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