DETERMINATION OF CHLOROTHALONIL IN WATER AND AGRICULTURAL PRODUCTS BY A MAGNETIC PARTICLE-BASED ENZYME-IMMUNOASSAY

被引：30

作者：

LAWRUK, TS ^{[1
]}

GUECO, AM ^{[1
]}

JOURDAN, SW ^{[1
]}

SCUTELLARO, AM ^{[1
]}

FLEEKER, JR ^{[1
]}

HERZOG, DP ^{[1
]}

RUBIO, FM ^{[1
]}

机构：

[1] N DAKOTA STATE UNIV,DEPT BIOCHEM,FARGO,ND 58105

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1995年 / 43卷 / 05期

关键词：

CHLOROTHALONIL; IMMUNOASSAY; WATER; PRODUCE; MAGNETIC PARTICLE; ELISA;

D O I：

10.1021/jf00053a052

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A competitive enzyme immunoassay for the quantitation of chlorothalonil in water and agricultural products was developed. This immunoassay utilizes amine-terminated superparamagnetic particles as the solid phase to covalently attach polyclonal rabbit anti-chlorothalonil antisera. Specificity studies indicate that the antibody can distinguish chlorothalonil from its major metabolites and structurally similar pesticides. The immunoassay has a detection limit of 0.07 parts per billion (ppb, ng/mL) in water. The assay compares favorably with GC measurements when water samples are analyzed (r = 0.984). Studies indicate that the assay can potentially be used to analyze agricultural products after homogenization with acetone (detection limit of 20 ppb for celery) or to analyze dislodgable residues after a methanol surface wash (detection limit of 5 ppb for snow pea pods).

引用

页码：1413 / 1419

页数：7