IDENTIFICATION OF HUMAN REMAINS BY AMPLIFICATION AND AUTOMATED SEQUENCING OF MITOCHONDRIAL-DNA

被引：104

作者：

SULLIVAN, KM

HOPGOOD, R

GILL, P

机构：

[1] Central Research and Support Establishment, The Forensic Science Service, Aldermaston, Reading, RG7 4PN, Berkshire

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1992年 / 105卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; SEQUENCING; MITOCHONDRIAL DNA; NONCODING REGION; HUMAN IDENTIFICATION;

D O I：

10.1007/BF02340829

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The highly decomposed remains of a corpse were identified by the amplification and direct sequencing of mitochondrial (mt) DNA. Degraded DNA was extracted from bone fragments and a necrotic skin sample and amplified at 2 hypervariable segments within the mitochondrial non-coding region using 2 rounds of nested PCR. Both strands of the amplified regions were sequenced and compared with each other to ensure fidelity of the data. Sequences from the bone and skin were found to be identical and matched data generated from a blood sample provided by a presumptive sister of the deceased. Thus, this evidence was consistent with the sister and the deceased being related.

引用

页码：83 / 86

页数：4

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