SCREENING BY PCR FOR DEFINED DNA-SEQUENCES IN MINIMAL AMOUNTS OF BARLEY TISSUE

被引：2

作者：

DEHMER, KJ

GRANER, A

WENZEL, G

机构：

[1] Biologische Bundesanstalt für Land‐ und Forstwirtschaft, Institut für Resistenzgenetik, Bockhorn, W-8059, Grünbach

来源：

PLANT BREEDING | 1991年 / 107卷 / 01期

关键词：

HORDEUM-VULGARE; PCR; DNA SCREENING; SELECTION;

D O I：

10.1111/j.1439-0523.1991.tb00529.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The potential use of the polymerase chain reaction (PCR) in screening plants for defined DNA sequences was demonstrated in barley (Hordeum vulgare). As templates for PCR, purified DNA as well as DNA from crude cell extracts of different barley tissues can be used. Specific synthesis of the target sequence was possible in both cases. Under optimal conditions, as little as 20 copies of the barley genome, i.e., 20 cells, were sufficient for successful amplification. This amount of DNA is equivalent to significantly less than 1 mg of fresh tissue.

引用

页码：70 / 72

页数：3

共 11 条

[1] PRIMARY STRUCTURE OF A B1 HORDEIN GENE FROM BARLEY [J].

BRANDT, A ;

MONTEMBAULT, A ;

CAMERONMILLS, V ;

RASMUSSEN, SK .

CARLSBERG RESEARCH COMMUNICATIONS, 1985, 50 (06) :333-345

[2] DETECTION OF SUBPICOGRAM QUANTITIES OF SPECIFIC DNA-SEQUENCES ON BLOT HYBRIDIZATION WITH BIOTINYLATED PROBES [J].

CHAN, VTW ;

FLEMING, KA ;

MCGEE, JOD .

NUCLEIC ACIDS RESEARCH, 1985, 13 (22) :8083-8091

[3] ASSESSMENT OF THE DEGREE AND THE TYPE OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IN BARLEY (HORDEUM-VULGARE) [J].

GRANER, A ;

SIEDLER, H ;

JAHOOR, A ;

HERRMANN, RG ;

WENZEL, G .

THEORETICAL AND APPLIED GENETICS, 1990, 80 (06) :826-832

[4] MOLECULAR ANALYSIS OF A MUTATION CONFERRING THE HIGH-LYSINE PHENOTYPE ON THE GRAIN OF BARLEY (HORDEUM-VULGARE) [J].

KREIS, M ;

SHEWRY, PR ;

FORDE, BG ;

RAHMAN, S ;

MIFLIN, BJ .

CELL, 1983, 34 (01) :161-167

[5] PRODUCTION OF DOUBLED HAPLOID LINES IN FREQUENCIES SUFFICIENT FOR BARLEY BREEDING PROGRAMS [J].

KUHLMANN, U ;

FOROUGHIWEHR, B .

PLANT CELL REPORTS, 1989, 8 (02) :78-81

[6]

Lassner M. W., 1989, PLANT MOL BIOL REP, V7, P116, DOI DOI 10.1007/BF02669627

[7] AMPLIFICATION AND ANALYSIS OF DNA-SEQUENCES IN SINGLE HUMAN-SPERM AND DIPLOID-CELLS [J].

LI, HH ;

GYLLENSTEN, UB ;

CUI, XF ;

SAIKI, RK ;

ERLICH, HA ;

ARNHEIM, N .

NATURE, 1988, 335 (6189) :414-417

[8]

ROLLO F, 1989, Journal of Genetics and Breeding, V43, P91

[9] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].

SAIKI, RK ;

SCHARF, S ;

FALOONA, F ;

MULLIS, KB ;

HORN, GT ;

ERLICH, HA ;

ARNHEIM, N .

SCIENCE, 1985, 230 (4732) :1350-1354

[10] DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS [J].

SOUTHERN, EM .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) :503-+

← 1 2 →