STRAIN DISCRIMINATION OF CULTURED AND SYMBIOTIC FRANKIA BY RFLP-PCR

被引：13

作者：

MCEWAN, NR

WHEELER, CT

MILNER, JJ

机构：

[1] Department of Botany, University of Glasgow, Glasgow

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 1994年 / 26卷 / 05期

关键词：

D O I：

10.1016/0038-0717(94)90240-2

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

A non-enzymic method is described for isolation of DNA from both cultured and symbiotic Frankia. Application of a combination of restriction enzyme digestion and electrophoresis techniques to a polymorphic fragment of the 16s rDNA gene, amplified by PCR, permitted discrimination between different Frankia genomic groups. The technique can be applied to both cultured Frankia and to identify Frankia in nodules.

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页码：541 / 545

页数：5

相关论文

共 9 条

[1] A SIMPLE METHOD FOR THE ISOLATION OF CHROMOSOMAL DNA FROM GRAM-POSITIVE OR ACID-FAST BACTERIA [J].

BOLLET, C ;

GEVAUDAN, MJ ;

DELAMBALLERIE, X ;

ZANDOTTI, C ;

DEMICCO, P .

NUCLEIC ACIDS RESEARCH, 1991, 19 (08) :1955-1955

[2] DEOXYRIBONUCLEIC-ACID RELATEDNESS AMONG MEMBERS OF THE GENUS FRANKIA [J].

FERNANDEZ, MP ;

MEUGNIER, H ;

GRIMONT, PAD ;

BARDIN, R .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (04) :424-429

[3] THE EFFECTIVITY OF FRANKIA FOR NODULATION AND NITROGEN-FIXATION IN ALNUS-RUBRA AND ALNUS-GLUTINOSA [J].

HOOKER, JE ;

WHEELER, CT .

PHYSIOLOGIA PLANTARUM, 1987, 70 (02) :333-341

[4] CATALOGING FRANKIA STRAINS [J].

LECHEVALIER, MP .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1983, 61 (11) :2964-2967

[5]

MANIATIS T, 1982, MOL CLONING

[6] PHYLOGENETIC-RELATIONSHIPS AMONG FRANKIA GENOMIC SPECIES DETERMINED BY USE OF AMPLIFIED 16S RDNA SEQUENCES [J].

NAZARET, S ;

COURNOYER, B ;

NORMAND, P ;

SIMONET, P .

JOURNAL OF BACTERIOLOGY, 1991, 173 (13) :4072-4078

[7] DETECTION AND ENUMERATION OF BACTERIA IN SOIL BY DIRECT DNA EXTRACTION AND POLYMERASE CHAIN-REACTION [J].

PICARD, C ;

PONSONNET, C ;

PAGET, E ;

NESME, X ;

SIMONET, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (09) :2717-2722

[8] IDENTIFICATION OF CASUARINA-FRANKIA STRAINS BY USE OF POLYMERASE CHAIN-REACTION (PCR) WITH ARBITRARY PRIMERS [J].

SELLSTEDT, A ;

WULLINGS, B ;

NYSTROM, U ;

GUSTAFSSON, P .

FEMS MICROBIOLOGY LETTERS, 1992, 93 (01) :1-5

[9] FRANKIA GENUS-SPECIFIC CHARACTERIZATION BY POLYMERASE CHAIN-REACTION [J].

SIMONET, P ;

GROSJEAN, MC ;

MISRA, AK ;

NAZARET, S ;

COURNOYER, B ;

NORMAND, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (11) :3278-3286