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STREPTOZOTOCIN-INDUCED GENOTOXIC EFFECTS IN CHINESE-HAMSTER CELLS - THE RESISTANT PHENOTYPE OF V79 CELLS

被引：11
作者：
CAPUCCI, MS
HOFFMANN, ME
NATARAJAN, AT
机构：
[1] UNIV CAMPINAS,INST BIOL,DEPT BIOQUIM,CAMPINAS,BRAZIL
[2] LEIDEN STATE UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,MGC,PRECLIN LABS,SYLVIUS LABS,2333 AL LEIDEN,NETHERLANDS
来源：
MUTATION RESEARCH LETTERS | 1995年 / 347卷 / 02期
关键词：
STREPTOZOTOCIN; METHYLNITROSOUREA; CHINESE HAMSTER CELLS; CHROMOSOMAL ABERRATION; SISTER-CHROMATID EXCHANGE; MUTATION;
D O I：
10.1016/0165-7992(95)90074-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The genotoxic effects of the methylating agent streptozotocin (STZ) on Chinese hamster cells CHO-9 and V79 were evaluated. The induction of cell killing, chromosomal aberrations, sister-chromatid exchanges (SCEs) and mutations was analyzed. Comparisons were made with the the STZ aglyconic analogue N-methyl-N-nitrosourea (MNU). V79 cells were found to be more resistant than CHO-9 cells to STZ and MNU killing effects, as well as to the induction of chromosomal aberrations and SCEs; however, V79 and CHO-9 cells appeared to be equally sensitive to the induction of 6-thioguanine resistant mutants by STZ. These results suggest that an error-free mechanism that tolerates DNA methylation damage confers a resistant phenotype to V79 cells to the genotoxic effects of methylation damage.
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页码：79 / 85
页数：7
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