MECHANISMS OF OVERLAP FORMATION IN NONHOMOLOGOUS DNA END JOINING

被引：99

作者：

PFEIFFER, P ^{[1
]}

THODE, S ^{[1
]}

HANCKE, J ^{[1
]}

VIELMETTER, W ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT NEUROSCI,LA JOLLA,CA 92093

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1994年 / 14卷 / 02期

关键词：

D O I：

10.1128/MCB.14.2.888

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rejoining of nonhomologous DNA termini plays a central role in processes of illegitimate recombination. In Xenopus egg extracts, DNA ends with noncomplementary il-nucleotide antiparallel single-strand protrusions are assumed to be joined by formation of short mismatched overlap intermediates. The extents of these overlaps may be set by single fortuitously matching base pairs and determine the patterns of subsequent gap filling and nick ligation. Under conditions of alternative overlap settings, rules for the most probable joining pathway and the effects of mismatches on junction formation were analyzed. We show that in certain cases fill-in and ligation converting overlap intermediates into covalently closed junctions may proceed in the presence of unrepaired mismatches, whereas in other cases, completion of junction formation is preceded by removal of mismatches. Results are discussed in relation with ''alignment'' proteins postulated tb structurally support overlap heteroduplexes during junction formation.

引用

页码：888 / 895

页数：8

共 27 条

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