DIFFERENTIATION OF HAEMONCHUS-PLACEI FROM H-CONTORTUS (NEMATODA, TRICHOSTRONGYLIDAE) BY THE RIBOSOMAL DNA 2ND INTERNAL TRANSCRIBED SPACER

被引：170

作者：

STEVENSON, LA ^{[1
]}

CHILTON, NB ^{[1
]}

GASSER, RB ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT VET SCI,WERRIBEE,VIC 3030,AUSTRALIA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1995年 / 25卷 / 04期

关键词：

HAEMONCHUS CONTORTUS; HAEMONCHUS PLACEI; RDNA; ITS-2; PCR-RFLP; SPECIES IDENTIFICATION;

D O I：

10.1016/0020-7519(94)00156-I

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

There has been much debate as to whether H. placei is a separate species to H. contortus. The aim of this study is to provide molecular information to assess the species status of H. placei. Using the polymerase chain reaction, the second internal transcribed spacer (ITS-2) of ribosomal DNA was amplified and sequenced. Comparison of the 231 base pair ITS-2 sequences showed no intraspecific variation in H. contortus, among one isolate from each of the United Kingdom. Switzerland and China and 5 isolates from within Australia, or among 3 Australian isolates of H. placei. Three (1.3%) nucleotide differences were detected between the ITS-2 sequences of H. contortus and H. placei. In addition, 2 diagnostic sites for the endonucleases BfaI and FokI allowed the delineation of H. placei from H. contortus. The data presented herein support previous morphological and genetic evidence that H. placei is a separate species to H. contortus.

引用

页码：483 / 488

页数：6

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