DECREASE IN AMBIENT [CL-] STIMULATES NITRIC-OXIDE RELEASE FROM CULTURED RAT MESANGIAL CELLS

被引:36
作者
TSUKAHARA, H
KRIVENKO, Y
MOORE, LC
GOLIGORSKY, MS
机构
[1] SUNY STONY BROOK, HLTH SCI CTR, DEPT MED, DIV NEPHROL, STONY BROOK, NY 11794 USA
[2] SUNY STONY BROOK, HLTH SCI CTR, DEPT PHYSIOL & BIOPHYS, STONY BROOK, NY 11794 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 267卷 / 01期
关键词
TUBULOGLOMERULAR FEEDBACK; CYTOSOLIC CALCIUM; AMPEROMETRIC NITRIC OXIDE SENSOR; NITRIC OXIDE SYNTHASE; CHLORIDE ION CONCENTRATION;
D O I
10.1152/ajprenal.1994.267.1.F190
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
It has been hypothesized that fluctuations of the ionic composition in the interstitium of juxtaglomerular apparatus (JGA) modulate the function of extraglomerular mesangial cells (MC), thereby participating in tubuloglomerular feedback (TGF) signal transmission. We examined the effects of isosmotic reductions in ambient sodium concentration ([Na+]) and [Cl-] on cytosolic calcium concentration ([Ca2+](i)) in cultured rat MC. Rapid reduction of [Na+] or [Cl-] in the bath induced a concentration-dependent rise in [Ca2+](i). MC are much more sensitive to decreases in ambient [Cl-] than to [Na+]; a decrease in [Cl-] as small as 14 mM was sufficient to elicit a detectable [Ca2+](i) response. These observations suggest that MC can be readily stimulated by modest perturbations of extracellular [Cl-]. Next, we examined whether activation of MC by lowered ambient [Cl-] influences cellular nitric oxide (NO) production. Using an amperometric NO sensor, we found that a 13 mM decrease in ambient [Cl-] caused a rapid, Ca2+/calmodulin-dependent rise in NO release from MC. This response was not inhibitable by dexamethasone, indicating the involvement of the constitutive rather than the inducible type of NO synthase in MC. In addition, the NO release was blunted by indomethacin pretreatment, suggesting that a metabolite(s) of cyclooxygenase regulates the activation of NO synthase in MC. Our findings that small perturbations in external [Cl-] stimulate MC to release NO, a highly diffusible and rapidly acting vasodilator, provide a possible mechanism to explain the transmission of the signal for the TGF response within the JGA.
引用
收藏
页码:F190 / F195
页数:6
相关论文
共 33 条
[21]   NITRIC-OXIDE ACTIVATES CYCLOOXYGENASE ENZYMES [J].
SALVEMINI, D ;
MISKO, TP ;
MASFERRER, JL ;
SEIBERT, K ;
CURRIE, MG ;
NEEDLEMAN, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7240-7244
[22]   ACTIVATION OF TUBULO-GLOMERULAR FEEDBACK BY CHLORIDE TRANSPORT [J].
SCHNERMANN, J ;
PLOTH, DW ;
HERMLE, M .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1976, 362 (03) :229-240
[23]   EFFECT OF INHIBITION OF PROSTAGLANDIN SYNTHESIS ON TUBULOGLOMERULAR FEEDBACK IN THE RAT-KIDNEY [J].
SCHNERMANN, J ;
SCHUBERT, G ;
HERMLE, M ;
HERBST, R ;
STOWE, NT ;
YARIMIZU, S ;
WEBER, PC .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1979, 379 (03) :269-279
[24]   LIBERATORS OF NO EXERT A DUAL EFFECT ON RENIN SECRETION FROM ISOLATED MOUSE RENAL JUXTAGLOMERULAR CELLS [J].
SCHRICKER, K ;
KURTZ, A .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (02) :F180-F186
[25]   AN ELECTROCHEMICAL MICROPROBE FOR DETECTING NITRIC-OXIDE RELEASE IN BRAIN-TISSUE [J].
SHIBUKI, K .
NEUROSCIENCE RESEARCH, 1990, 9 (01) :69-76
[26]   CA-2+ CALMODULIN-REGULATED NITRIC-OXIDE SYNTHASES [J].
SHMIDT, HHHW ;
POLLOCK, JS ;
NAKANE, M ;
FORSTERMANN, U ;
MURAD, F .
CELL CALCIUM, 1992, 13 (6-7) :427-434
[27]   SYNTHESIS AND ACTION OF NITRIC-OXIDE IN RAT GLOMERULAR MESANGIAL CELLS [J].
SHULTZ, PJ ;
TAYEH, MA ;
MARLETTA, MA ;
RAIJ, L .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (04) :F600-F606
[28]  
SPRINGALL D, 1991, Journal of the American Society of Nephrology, V2, P512
[29]   MEASUREMENT OF CYTOSOLIC FREE CA-2+ IN INDIVIDUAL SMALL CELLS USING FLUORESCENCE MICROSCOPY WITH DUAL EXCITATION WAVELENGTHS [J].
TSIEN, RY ;
RINK, TJ ;
POENIE, M .
CELL CALCIUM, 1985, 6 (1-2) :145-157
[30]   CONTINUOUS MONITORING OF NITRIC-OXIDE RELEASE FROM HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS [J].
TSUKAHARA, H ;
GORDIENKO, DV ;
GOLIGORSKY, MS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 193 (02) :722-729