EFFECTS OF CAPSAICIN ON RAT-LIVER S9-MEDIATED METABOLISM AND DNA-BINDING OF AFLATOXIN

被引：45

作者：

TEEL, RW

机构：

[1] Department of Physiology and Pharmacology, Toma Linda, University School of Medicine, Loma Linda, CH

来源：

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL | 1991年 / 15卷 / 01期

关键词：

D O I：

10.1080/01635589109514108

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

At concentrations of 25, 50, and 100-mu-M, capsaicin, which is the major component in various aspects of Capsicum hot peppers, decreased the binding of aflatoxin (AFB1) to calf thymus DNA by 19%, 44%, and 71%, respectively, in incubations with rat liver S9. At concentrations of 50 and 100-mu-M, capsaicin decreased the formation of AFB-DNA adducts (AFB1-N7-Gua) by 53% and 75% as determined by high-pressure liquid chromatography (HPLC). HPLC analysis of organosoluble fractions showed that these effects correlated with a concentration-dependent decrease in S9-mediated metabolism of AFB1 by capsaicin. Capsaicin also altered the formation of water-soluble conjugates of AFB1. This was indicated by a decrease in radioactivity in water-soluble fractions and in glutathione conjugates of AFB1 analyzed by HPLC. These results suggest that capsaicin inhibited the biotransformation of AFB1 by modifying Phase I hepatic enzyme activity.

引用

页码：27 / 32

页数：6

共 26 条

[11]

Guengerich F.P., Roles of Cytochrome P-450 Enzymes in Chemical Carcinogenesis and Cancer Chemotherapy, Cancer Res, 48, pp. 2946-2954, (1988)

[12]

Maron D.M., Ames B., Revised Methods for the Salmonella Mutagenicity Test, Mutat Res, 113, pp. 173-215, (1983)

[13]

Lowry D.H., Rosebrough N.J., Farr A.L., Randall R.J., Protein Measurement With the Folin Phenol Reagent, J Biol Chem, 193, pp. 265-275, (1951)

[14]

Lotlikar P.D., Raj H.G., Bohm L.S., Ho L.L., Jhee E.C., Et al., A Mechanism of Inhibition of Aflatoxin BrDNA Binding in the Liver by Phenobarbital Treatment of Rats, Cancer Res, 49, pp. 951-957, (1989)

[15]

Kensler T.W., Egner P.A., Davidson N.E., Roebuck B.D., Pikul A., Et al., Modulation of Aflatoxin Metabolism, Aflatoxin-N7-guanine Formation and Hepatic Tumorigenesis in Rats Fed Ethoxyquin: Rate of Induction of Glutathione S-Transferases, Cancer Res, 46, pp. 3924-3931, (1986)

[16]

Maniatus T., Fritsch E.F., Sambrooks J., Molecular Cloning: A Laboratory Manual, (1982)

[17]

Burton K.A., Study of the Conditions and Mechanisms of the Diphenylamine Reaction for the Colorimetric Estimation of Deoxyribonucleic Acid, Biochemistry, 162, pp. 315-323, (1950)

[18]

Mandal S., Ahuja A., Shivapurkur N.M., Cheng S., Groopman J.D., Et al., Inhibition of Aflatoxin B, Mutagenesis in Salmonella typhimurium and DNA Damage in Cultured Rat and Human Tracheobronchial Tissues by Ellagic Acid, Carcinogenesis, 8, pp. 1651-1656, (1987)

[19]

Campbell T.G., Hayes J.R., The Role of Aflatoxin Metabolism in its Toxic Lesion, Toxicol Appl Pharmacol, 35, pp. 199-222, (1976)

[20]

Coulombe R.A., Shelton D.W., Sinnhuber R.O., Nixon J.E., Comparative Mutagenicity of Aflatoxins Using a Salmonella/Trout Hepatic Enzyme Activation System, Carcinogenesis 3, pp. 1261-1264, (1982)

← 1 2 3 →