CHARACTERIZATION OF BEET CURLY TOP VIRUS SUBGENOMIC DNA LOCALIZES SEQUENCES REQUIRED FOR REPLICATION

被引：36

作者：

FRISCHMUTH, T ^{[1
]}

STANLEY, J ^{[1
]}

机构：

[1] JOHN INNES INST,JOHN INNES CTR PLANT SCI RES,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

VIROLOGY | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0042-6822(92)90612-S

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Subgenomic viral DNA is accumulated in Nicotiana benthamiana and Beta vulgaris plants agroinoculated with the geminivirus beet curly top virus. The subgenomic DNA is more abundant in N. benthamiana and is distributed between two broad size groups in this host. Six unique examples, ranging in size from 887 to 1311 nucleotides, have been cloned from viral double-stranded DNA purified from N. benthamiana and analyzed by sequence determination. Deletions are distributed throughout most of the genome and only nucleotides 2946-410 are represented in all subgenomic DNAs. Comparison with a previously characterized subgenomic DNA suggests that cis-acting signals necessary for viral DNA replication are located in a predominantly intergenic region between nucleotides 2946-308. © 1992.

引用

页码：808 / 811

页数：4

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