ACYLATION OF SUBTILISIN WITH LONG FATTY ACYL RESIDUES AFFECTS ITS ACTIVITY AND THERMOSTABILITY IN AQUEOUS-MEDIUM

被引：10

作者：

PLOU, FJ

BALLESTEROS, A

机构：

[1] Departamento de Biocatalisis, Instituto de Catálisis, CSIC

来源：

FEBS LETTERS | 1994年 / 339卷 / 1-2期

关键词：

SUBTILISIN CARLSBERG; CHEMICAL MODIFICATION; ENZYME HYDROPHOBICITY; IN VITRO HYDROPHOBIZATION; ENZYME STABILIZATION;

D O I：

10.1016/0014-5793(94)80415-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Subtilisin Carlsberg has been artificially hydrophobized by acylation with octanoyl or palmitoyl chlorides. Samples with several degrees of substitution were obtained. Hydrophobization facilitates in some cases the binding of synthetic or natural substrates. Furthermore, derivatized subtilisins show improved thermal stability (15-fold at 45 degrees C) in aqueous solution. As a result, octanoyl-subtilisin exhibits enhanced thermostability without losing biological activity.

引用

页码：200 / 204

页数：5

共 30 条

[1] REDUCTIVE ALKYLATION OF LIPASE [J].

AMPON, K ;

SALLEH, AB ;

SALAM, F ;

YUNUS, WMZW ;

RAZAK, CNA ;

BASRI, M .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (07) :597-601

[2] INDUSTRIAL ENZYMOLOGY - A LOOK TOWARDS THE FUTURE [J].

ARBIGE, MV ;

PITCHER, WH .

TRENDS IN BIOTECHNOLOGY, 1989, 7 (12) :330-335

[3]

BARDSLEY GW, 1993, PACKAGE SIMULATION C

[4] DETERMINATION OF CONCENTRATION OF HYDROLYTIC ENZYME SOLUTIONS - ALPHA-CHYMOTRYPSIN TRYPSIN PAPAIN ELASTASE SUBTILISIN AND ACETYLCHOLINESTERASE [J].

BENDER, ML ;

BEGUECAN.ML ;

BLAKELEY, RL ;

BRUBACHER, LJ ;

FEDER, J ;

GUNTER, CR ;

KEZDY, FJ ;

KILLHEFFER, JV ;

MARSHALL, TH ;

MILLER, CG ;

ROESKE, RW ;

STOOPS, JK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (24) :5890-+

[5] MEASUREMENT OF AMINO GROUPS IN PROTEINS AND PEPTIDES [J].

FIELDS, R .

BIOCHEMICAL JOURNAL, 1971, 124 (03) :581-&

[6] ACTIVITY OF LIPASE IN WATER-IN-OIL MICROEMULSIONS [J].

FLETCHER, PDI ;

ROBINSON, BH ;

FREEDMAN, RB ;

OLDFIELD, C .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1985, 81 :2667-2679

[7] IMMOBILIZATION OF PROTEIN MOLECULES ON LIPOSOMES - ANCHORAGE BY ARTIFICIALLY BOUND UNSATURATED HYDROCARBON TAILS [J].

GOLDMACHER, VS .

BIOCHEMICAL PHARMACOLOGY, 1983, 32 (07) :1207-1210

[8]

JOHN RA, 1992, ENZYME ASSAYS, P82

[9] LIPID MODIFICATION OF PROTEINS AND THEIR MEMBRANE-TRANSPORT [J].

KABANOV, AV ;

LEVASHOV, AV ;

ALAKHOV, VY .

PROTEIN ENGINEERING, 1989, 3 (01) :39-42

[10] FATTY-ACID ACYLATED ANTIBODIES AGAINST VIRUS SUPPRESS ITS REPRODUCTION IN CELLS [J].

KABANOV, AV ;

OVCHARENKO, AV ;

MELIKHUBAROV, NS ;

BANNIKOV, AI ;

ALAKHOV, VY ;

KISELEV, VI ;

SVESHNIKOV, PG ;

KISELEV, OI ;

LEVASHOV, AV ;

SEVERIN, ES .

FEBS LETTERS, 1989, 250 (02) :238-240

← 1 2 3 →