The messenger RNAs coding for vasopressin, oxytocin, luteinizing hormone releasing-hormone and somatostatin have been detected in tissue sections of the rat brain, especially in the hypothalamus with radioactive and biotinylated oligonucleotide probes. The results demonstrate that neuropeptide mRNAs are present in the cytoplasm of cell bodies, in processes and in punctate structures in the vicinity of the cell bodies. These results demonstrate that neuropeptide mRNAs can be transported outside the cell body most probably in proximal dendrites but also in some of their branching, and possibly at synaptic contacts. These data suggest that neuropeptide mRNA could undergo a specific compartmentation that could contribute to the targetting of the corresponding peptide inside neurons. © 1990.
