MUTATIONAL ANALYSIS OF SEGMENTAL STABILIZATION OF TRANSCRIPTS FROM THE ZYMOMONAS-MOBILIS GAP-PGK OPERON

被引:13
作者
BURCHHARDT, G [1 ]
KESHAV, KF [1 ]
YOMANO, L [1 ]
INGRAM, LO [1 ]
机构
[1] UNIV FLORIDA,DEPT MICROBIOL & CELL SCI,GAINESVILLE,FL 32611
关键词
D O I
10.1128/JB.175.8.2327-2333.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In Zymomonas mobilis, the genes encoding glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase are transcribed together from the gap-pgk operon. However, higher levels of the former enzyme are present in the cytoplasm because of increased stability of a 5' segment containing the gap coding region. This segment is bounded by an upstream untranslated region which can be folded into many stem-loop structures and a prominent intercistronic stem-loop. Mutations eliminating a proposed stem-loop in the untranslated region or the intercistronic stem-loop resulted in a decrease in the stability and pool size of the 5' gap segment. Site-specific mutations in the unpaired regions of both of these stems also altered the message pools. Elimination of the intercistronic stem appeared to reduce the endonucleolytic cleavage within the pgk coding region, increasing the stability and abundance of the full-length message. DNA encoding the prominent stem-loop at the 3' end of the message was shown to be a transcriptional terminator both in Z. mobilis and in Escherichia coli. This third stem-loop region (part of the transcriptional terminator) was required to stabilize the full-length gap-pgk message.
引用
收藏
页码:2327 / 2333
页数:7
相关论文
共 40 条
[21]   A 5'-TERMINAL STEM LOOP STRUCTURE CAN STABILIZE MESSENGER-RNA IN ESCHERICHIA-COLI [J].
EMORY, SA ;
BOUVET, P ;
BELASCO, JG .
GENES & DEVELOPMENT, 1992, 6 (01) :135-148
[22]   THE STABILITY OF BACTERIOPHAGE-T4 GENE-32 MESSENGER-RNA - A 5' LEADER SEQUENCE THAT CAN STABILIZE MESSENGER-RNA TRANSCRIPTS [J].
GORSKI, K ;
ROCH, JM ;
PRENTKI, P ;
KRISCH, HM .
CELL, 1985, 43 (02) :461-469
[23]  
KENNELL DE, 1986, MAXIMIZING GENE EXPR, P101
[24]   BIOLOGICAL CONSEQUENCES OF SEGMENTAL ALTERATIONS IN MESSENGER-RNA STABILITY - EFFECTS OF DELETION OF THE INTERCISTRONIC HAIRPIN LOOP REGION OF THE RHODOBACTER-CAPSULATUS-PUF OPERON [J].
KLUG, G ;
ADAMS, CW ;
BELASCO, J ;
DOERGE, B ;
COHEN, SN .
EMBO JOURNAL, 1987, 6 (11) :3515-3520
[25]  
KORAIMANN G, 1989, NUCLEIC ACIDS RES, V17, P1283
[26]  
Luria SE, 1943, GENETICS, V28, P491
[27]   EXPRESSION OF ZYMOMONAS-MOBILIS ADHB (ENCODING ALCOHOL DEHYDROGENASE-II) AND ADHB-LACZ OPERON FUSIONS IN RECOMBINANT ZYMOMONAS-MOBILIS [J].
MACKENZIE, KF ;
CONWAY, T ;
ALDRICH, HC ;
INGRAM, LO .
JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4577-4582
[28]   CONFORMATIONAL ALTERATIONS IN THE ERMC TRANSCRIPT INVIVO DURING INDUCTION [J].
MAYFORD, M ;
WEISBLUM, B .
EMBO JOURNAL, 1989, 8 (13) :4307-4314
[29]   MESSENGER-RNA DEGRADATION BY PROCESSIVE 3'-5' EXORIBONUCLEASES INVITRO AND THE IMPLICATIONS FOR PROKARYOTIC MESSENGER-RNA DECAY INVIVO [J].
MCLAREN, RS ;
NEWBURY, SF ;
DANCE, GSC ;
CAUSTON, HC ;
HIGGINS, CF .
JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (01) :81-95
[30]   COORDINATION OF EXPRESSION OF ZYMOMONAS-MOBILIS GLYCOLYTIC AND FERMENTATIVE ENZYMES - A SIMPLE HYPOTHESIS BASED ON MESSENGER-RNA STABILITY [J].
MEJIA, JP ;
BURNETT, ME ;
AN, HJ ;
BARNELL, WO ;
KESHAV, KF ;
CONWAY, T ;
INGRAM, LO .
JOURNAL OF BACTERIOLOGY, 1992, 174 (20) :6438-6443