SEPARATION AND CHARACTERIZATION OF RICE PROTEINS

被引：85

作者：

TSUGITA, A

KAWAKAMI, T

UCHIYAMA, Y

KAMO, M

MIYATAKE, N

NOZU, Y

机构：

[1] SCI UNIV TOKYO, DEPT APPL BIOL SCI, NODA, CHIBA, JAPAN

[2] SCI UNIV TOKYO, FAC PHARMACEUT SCI, SHINJUKU KU, TOKYO, JAPAN

[3] NATL INST AGROBIOL RESOURCES, TSUKUBA, IBARAKI, JAPAN

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 05期

关键词：

D O I：

10.1002/elps.1150150198

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus) were isolated and then separated by two-dimensional gel electrophoresis (2-DE). The protein spots were characterized according to molecular weight, isoelectric point and partial amino-terminal sequence. Electrophoresis was carried out by isoelectric focusing (IEF), nonequilibrium pH gradient electrophoresis (NEPHGE) and immobilized pH gradient (IPG) in the first dimension, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension. With the aid of nine marker proteins, the patterns of IEF, NEPHGE and IPG 2-DE gels were graphically combined by computer into a single synthetic image for each tissue, respectively, and these images for the nine tissues and one organelle were again combined into a single 2-DE image for the integrated rice protein spots. The rice 2-DE gel image resolved 4892 proteins. About 3% of the spots are characterized by amino-terminal sequencing.

引用

页码：708 / 720

页数：13

共 41 条

[11]

EBATA N, 1991, Patent No. 5064768

[12] MOUSE-LIVER PROTEIN DATABASE - A CATALOG OF PROTEINS DETECTED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS [J].

GIOMETTI, CS ;

TAYLOR, J ;

TOLLAKSEN, SL .

ELECTROPHORESIS, 1992, 13 (12) :970-991

[13]

HEWICK RM, 1981, J BIOL CHEM, V256, P7990

[14] RICE CHLOROPLAST DNA - A PHYSICAL MAP AND THE LOCATION OF THE GENES FOR LARGE SUBUNIT OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE AND THE 32 KD PHOTOSYSTEM-II REACTION CENTER PROTEIN [J].

HIRAI, A ;

ISHIBASHI, T ;

MORIKAMI, A ;

IWATSUKI, N ;

SHINOZAKI, K ;

SUGIURA, M .

THEORETICAL AND APPLIED GENETICS, 1985, 70 (02) :117-122

[15] THE COMPLETE SEQUENCE OF THE RICE (ORYZA-SATIVA) CHLOROPLAST GENOME - INTERMOLECULAR RECOMBINATION BETWEEN DISTINCT TRANSFER-RNA GENES ACCOUNTS FOR A MAJOR PLASTID DNA INVERSION DURING THE EVOLUTION OF THE CEREALS [J].

HIRATSUKA, J ;

SHIMADA, H ;

WHITTIER, R ;

ISHIBASHI, T ;

SAKAMOTO, M ;

MORI, M ;

KONDO, C ;

HONJI, Y ;

SUN, CR ;

MENG, BY ;

LI, YQ ;

KANNO, A ;

NISHIZAWA, Y ;

HIRAI, A ;

SHINOZAKI, K ;

SUGIURA, M .

MOLECULAR & GENERAL GENETICS, 1989, 217 (2-3) :185-194

[16]

KAMO M, 1989, Protein Sequences and Data Analysis, V2, P289

[17]

KANEMATSU S, 1990, PLANT CELL PHYSIOL, V31, P99

[18] THE PRESENCE OF AN ENZYME THAT CONVERTS INDOLE-3-ACETAMIDE INTO IAA IN WILD AND CULTIVATED RICE [J].

KAWAGUCHI, M ;

KOBAYASHI, M ;

SAKURAI, A ;

SYONO, K .

PLANT AND CELL PHYSIOLOGY, 1991, 32 (02) :143-149

[19]

Kawakami Takao, 1993, Protein Sequences and Data Analysis, V5, P269

[20]

KISHIMOTO N, 1992, RICE GENET NEWSL, V9, P118

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