CONVENTIONAL IMMUNOASSAYS UNDERESTIMATE ANTI-GST ANTIBODY TITER

被引：13

作者：

CARTWRIGHT, GA

ROTHEL, JS

LIGHTOWLERS, MW

机构：

[1] Molecular Parasitology Unit, Faculty of Veterinary Science, Veterinary Clinical Centre, Werribee, Vic. 3030, Princes Highway

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 179卷 / 01期

基金：

英国医学研究理事会;

关键词：

ELISA; GLUTATHIONE S-TRANSFERASE; FUSION PROTEIN; IMMUNOASSAY; RECOMBINANT PROTEIN;

D O I：

10.1016/0022-1759(94)00267-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper highlights problems associated with the quantitation of serum antibody levels to recombinant glutathione S-transferase (GST). Measurement of anti-GST antibodies in conventional immunoassays, where GST is bound directly to the ELISA plate, was found to substantially underestimate the amount of GST-specific antibody levels in test sera. This insensitivity in immunoassay of anti-GST antibodies can be overcome by using any one of several recombinant GST fusion proteins as the coating antigen in ELISA rather than simply GST. Comparison of anti-GST antibody titres assessed by the two procedures indicated that use of unfused GST underestimated the anti-GST antibody titre by more than ten-fold.

引用

页码：31 / 35

页数：5

共 11 条

[1] MOLECULAR-CLONING OF A PROTECTIVE ANTIGEN OF SCHISTOSOMES [J].

BALLOUL, JM ;

SONDERMEYER, P ;

DREYER, D ;

CAPRON, M ;

GRZYCH, JM ;

PIERCE, RJ ;

CARVALLO, D ;

LECOCQ, JP ;

CAPRON, A .

NATURE, 1987, 326 (6109) :149-153

[2]

Bos E S, 1981, J Immunoassay, V2, P187, DOI 10.1080/15321818108056977

[3] A RECOMBINANT ANTIGEN WITH POTENTIAL FOR SERODIAGNOSIS OF ECHINOCOCCUS-GRANULOSUS INFECTION IN DOGS [J].

GASSER, RB ;

LIGHTOWLERS, MW ;

RICKARD, MD .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1990, 20 (07) :943-950

[4] VACCINATION AGAINST TAENIA-TAENIAEFORMIS INFECTION IN RATS USING A RECOMBINANT PROTEIN AND PRELIMINARY-ANALYSIS OF THE INDUCED ANTIBODY-RESPONSE [J].

ITO, A ;

BOGH, HO ;

LIGHTOWLERS, MW ;

MITCHELL, GF ;

TAKAMI, T ;

KAMIYA, M ;

ONITAKE, K ;

RICKARD, MD .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 44 (01) :43-49

[5] VACCINATION AGAINST OVINE CYSTICERCOSIS USING A DEFINED RECOMBINANT ANTIGEN [J].

JOHNSON, KS ;

HARRISON, GBL ;

LIGHTOWLERS, MW ;

OHOY, KL ;

COUGLE, WG ;

DEMPSTER, RP ;

LAWRENCE, SB ;

VINTON, JG ;

HEATH, DD ;

RICKARD, MD .

NATURE, 1989, 338 (6216) :585-587

[6] ASSESSMENT OF THE PREVALENCE AND TITER OF ANTIBODIES TO A CANDIDATE SCHISTOSOMIASIS VACCINE MOLECULE, SJ26, IN SEVERAL HUMAN-SERUM BANKS [J].

LIGHTOWLERS, MW ;

MITCHELL, GF .

ACTA TROPICA, 1989, 46 (04) :229-238

[7] DETECTION OF ANTIBODIES TO CAPRINE ARTHRITIS-ENCEPHALITIS VIRUS USING RECOMBINANT GAG PROTEINS [J].

RIMSTAD, E ;

EAST, N ;

DEROCK, E ;

HIGGINS, J ;

PEDERSEN, NC .

ARCHIVES OF VIROLOGY, 1994, 134 (3-4) :345-356

[8]

SEXTON JL, 1994, J IMMUNOL, V152, P1861

[9] SINGLE-STEP PURIFICATION OF POLYPEPTIDES EXPRESSED IN ESCHERICHIA-COLI AS FUSIONS WITH GLUTATHIONE S-TRANSFERASE [J].

SMITH, DB ;

JOHNSON, KS .

GENE, 1988, 67 (01) :31-40

[10] THE DEVELOPMENT OF A RECOMBINANT BABESIA VACCINE [J].

WRIGHT, IG ;

CASU, R ;

COMMINS, MA ;

DALRYMPLE, BP ;

GALE, KR ;

GOODGER, BV ;

RIDDLES, PW ;

WALTISBUHL, DJ ;

ABETZ, I ;

BERRIE, DA ;

BOWLES, Y ;

DIMMOCK, C ;

HAYES, T ;

KALNINS, H ;

LEATCH, G ;

MCCRAE, R ;

MONTAGUE, PE ;

NISBET, IT ;

PARRODI, F ;

PETERS, JM ;

SCHEIWE, PC ;

SMITH, W ;

RODEBRAMANIS, K ;

WHITE, MA .

VETERINARY PARASITOLOGY, 1992, 44 (1-2) :3-13

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