METAL RECOGNITION BY IN-VITRO SELECTION

被引：3

作者：

GUPTA, K ^{[1
]}

YIN, J ^{[1
]}

机构：

[1] DARTMOUTH COLL,THAYER SCH ENGN,HANOVER,NH 03755

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 45卷 / 05期

关键词：

MOLECULAR RECOGNITION; IN VITRO SELECTION; METAL-BINDING PROTEIN; MOLECULAR PROCESS ENGINEERING;

D O I：

10.1002/bit.260450512

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We propose an in vitro selection strategy to identify bacteriophage variants that recognize metal ions in solution. In 6 M urea, phage T7 loses 99.9% of its activity in less than 5 min. Inactivation is accelerated by gold, but slowed by zinc and magnesium. Selection of phage over five generations in the presence of gold, zinc, and magnesium increases phage half-lives 4-, 10-, and 70-fold, respectively. As selections are repeated, phage become increasingly dependent on the specific metal employed in the selection; indicating the suitability of the strategy for optimization of metal-ion recognition. (C) 1995 John Wiley and Sons, Inc.

引用

页码：458 / 462

页数：5

共 32 条

[1] DIRECTED EVOLUTION OF NUCLEIC-ACIDS BY INDEPENDENT REPLICATION AND SELECTION [J].