DIRECT SELECTION OF ANTIBODIES THAT COORDINATE METALS FROM SEMISYNTHETIC COMBINATORIAL LIBRARIES

被引：95

作者：

BARBAS, CF ^{[1
]}

ROSENBLUM, JS ^{[1
]}

LERNER, RA ^{[1
]}

机构：

[1] SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 14期

关键词：

METALLOPROTEINS; CATALYTIC ANTIBODIES; SURFACE EXPRESSION; MAGNETITE;

D O I：

10.1073/pnas.90.14.6385

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An iterative strategy for the selection of catalytic metalloantibodies is described. The first stage of this strategy is validated by the selection of semisynthetic antibodies that coordinate a variety of different metal ions and the metal oxide magnetite. These results have implications not only for the development of catalytic metalloantibodies but also for the development of reagents for magnetic resonance imaging, delivery of radioisotopes, and purification of recombinant proteins.

引用

页码：6385 / 6389

页数：5

共 29 条

[1]

ADAM ET, 1991, ADV PROTEIN CHEM, V42, P145

[2]

BAKER BL, 1978, J BIOL CHEM, V253, P8444

[3] SEMISYNTHETIC COMBINATORIAL ANTIBODY LIBRARIES - A CHEMICAL SOLUTION TO THE DIVERSITY PROBLEM [J].

BARBAS, CF ;

BAIN, JD ;

HOEKSTRA, DM ;

LERNER, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4457-4461

[4] ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE [J].

BARBAS, CF ;

KANG, AS ;

LERNER, RA ;

BENKOVIC, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :7978-7982

[5]

BARBAS CF, 1991, METHODS COMPANION ME, V2, P119

[6] PB(II)-CATALYSED CLEAVAGE OF THE SUGAR-PHOSPHATE BACKBONE OF YEAST TRANSFER RNAPHE - IMPLICATIONS FOR LEAD TOXICITY AND SELF-SPLICING RNA [J].

BROWN, RS ;

HINGERTY, BE ;

DEWAN, JC ;

KLUG, A .

NATURE, 1983, 303 (5917) :543-546

[7] ENGINEERED IRON OXIDE-ADHESION MUTANTS OF THE ESCHERICHIA-COLI PHAGE-LAMBDA RECEPTOR [J].

BROWN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (18) :8651-8655

[8] NICKEL(III)-PROMOTED DNA CLEAVAGE WITH AMBIENT DIOXYGEN [J].

CHENG, CC ;

ROKITA, SE ;

BURROWS, CJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1993, 32 (02) :277-278

[9] DNA-SCISSION AND PROTEIN-SCISSION ACTIVITIES OF ASCORBATE IN THE PRESENCE OF COPPER-ION AND A COPPER-PEPTIDE COMPLEX [J].

CHIOU, SH .

JOURNAL OF BIOCHEMISTRY, 1983, 94 (04) :1259-1267

[10] CANONICAL STRUCTURES FOR THE HYPERVARIABLE REGIONS OF IMMUNOGLOBULINS [J].

CHOTHIA, C ;

LESK, AM .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (04) :901-917

← 1 2 3 →